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Literature summary for 1.5.1.3 extracted from

  • Haber, D.A.; Beverley, S.M.; Kiely, M.L.; Schimke, R.T.
    Properties of an altered dihydrofolate reductase encoded by amplified genes in cultured mouse fibroblasts (1981), J. Biol. Chem., 256, 9501-9510.
    View publication on PubMed

Protein Variants

Protein Variants Comment Organism
additional information selection of cell line with altered enzyme due to decreased methotrexate-sensitivity Mus musculus

Inhibitors

Inhibitors Comment Organism Structure
methasquin
-
Mus musculus
methotrexate wild-type Mus musculus
pyrimethamine
-
Mus musculus
trimethoprim folate antagonist drug Mus musculus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0022
-
NADPH altered enzyme Mus musculus
0.003
-
7,8-dihydrofolate wild-type enzyme Mus musculus
0.01
-
7,8-dihydrofolate altered enzyme Mus musculus
0.03
-
NADPH wild-type Mus musculus

Organism

Organism UniProt Comment Textmining
Mus musculus
-
altered enzyme encoded by amplified genes in cultured fibroblasts
-

Source Tissue

Source Tissue Comment Organism Textmining
fibroblast cultured 3T6 mouse embryo fibroblasts Mus musculus
-

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
additional information
-
1 mM NADPH protects against inactivation Mus musculus
55
-
half-life: 8 min Mus musculus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.243
-
7,8-dihydrofolate altered enzyme Mus musculus
4.67
-
7,8-dihydrofolate wild-type enzyme Mus musculus