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Literature summary for 1.5.1.3 extracted from
- Soluble expression in Escherichia coli, one-step purification, and characterization of Chinese hamster dihydrofolate reductase (1997), Protein Expr. Purif., 9, 246-252.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| guanidinium hydrochloride | recombinant enzyme, 2.5fold activation at 0.5 M | Cricetulus griseus |  |
| Urea | recombinant enzyme, 4fold activation at 3 M | Cricetulus griseus | |
Application
| Application | Comment | Organism |
|---|---|---|
| medicine | enzyme is a target of several anti-folate inhibitory drugs to combat bacteria, protozoa and cancer | Cricetulus griseus |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| overexpression in Escherichia coli, regulation by temperature-sensitive repressor of the promotor | Cricetulus griseus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| methotrexate | - |
Cricetulus griseus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00048 | - |
7,8-dihydrofolate | recombinant enzyme | Cricetulus griseus | |
| 0.00133 | - |
NADPH | recombinant enzyme | Cricetulus griseus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| soluble | recombinant enzyme from Escherichia coli | Cricetulus griseus | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 23000 | - |
recombinant enzyme from E. coli, SDS-PAGE and DNA-sequence determination | Cricetulus griseus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Cricetulus griseus | - |
- |
- |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 4.7 | - |
purified, recombinant enzyme | Cricetulus griseus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 7,8-dihydrofolate + NADPH | enzyme plays important role in nucleotide biosynthesis | Cricetulus griseus | 5,6,7,8-tetrahydrofolate + NADP+ | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | - |
recombinant enzyme, assay at | Cricetulus griseus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.65 | - |
7,8-dihydrofolate | recombinant enzyme | Cricetulus griseus | |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0000014 | - |
methotrexate | recombinant enzyme | Cricetulus griseus | |
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