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Literature summary for 1.5.1.3 extracted from
- Dihydrofolate reductase and thymidylate synthase in plants: An open problem (1993), Physiol. Plant., 88, 509-521.
No PubMed abstract available
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| additional information | - |
Crithidia fasciculata | |
| additional information | overview antifolate inhibitors, especially methotrexate, and sensitivity or resistance in plants | Datura inoxia | |
| additional information | overview antifolate inhibitors, especially methotrexate, and sensitivity or resistance in plants | Daucus carota | |
| additional information | overview antifolate inhibitors, especially methotrexate, and sensitivity or resistance in plants | Glycine max | |
| additional information | overview antifolate inhibitors, especially methotrexate, and sensitivity or resistance in plants | Nicotiana plumbaginifolia | |
| additional information | overview antifolate inhibitors, especially methotrexate, and sensitivity or resistance in plants | Nicotiana tabacum | |
| additional information | overview antifolate inhibitors, especially methotrexate, and sensitivity or resistance in plants | Petunia x hybrida | |
| additional information | - |
Tetradesmus obliquus | |
| additional information | overview antifolate inhibitors, especially methotrexate, and sensitivity or resistance in plants | Zea mays |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | - |
Leishmania tropica | |
| additional information | - |
additional information | overview | Tetradesmus obliquus | |
| additional information | - |
additional information | overview | Pisum sativum | |
| additional information | - |
additional information | overview | Glycine max | |
| additional information | - |
additional information | overview | Crithidia fasciculata | |
| additional information | - |
additional information | overview | Daucus carota | |
| additional information | - |
additional information | overview | Oryza sativa |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| additional information | - |
overview | Tetradesmus obliquus |
| additional information | - |
overview | Glycine max |
| additional information | - |
overview | Crithidia fasciculata |
| additional information | - |
overview | Daucus carota |
| additional information | - |
overview | Leishmania tropica |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Crithidia fasciculata | - |
- |
- |
| Datura inoxia | - |
- |
- |
| Daucus carota | - |
- |
- |
| Euglena gracilis | - |
- |
- |
| Glycine max | - |
- |
- |
| Leishmania tropica | - |
- |
- |
| Nicotiana plumbaginifolia | - |
- |
- |
| Nicotiana tabacum | - |
- |
- |
| Oryza sativa | - |
- |
- |
| Petunia x hybrida | - |
- |
- |
| Pisum sativum | - |
- |
- |
| Tetradesmus obliquus | - |
- |
- |
| Zea mays | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| overview | Tetradesmus obliquus |
| overview | Glycine max |
| overview | Daucus carota |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| 5,6,7,8-tetrahydrofolate + NADP+ = 7,8-dihydrofolate + NADPH + H+ | bifunctional proteins with DHFR and thymidylate synthase activity | Tetradesmus obliquus | |
| 5,6,7,8-tetrahydrofolate + NADP+ = 7,8-dihydrofolate + NADPH + H+ | bifunctional proteins with DHFR and thymidylate synthase activity | Crithidia fasciculata | |
| 5,6,7,8-tetrahydrofolate + NADP+ = 7,8-dihydrofolate + NADPH + H+ | bifunctional proteins with DHFR and thymidylate synthase activity | Daucus carota |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| seedling | - |
Pisum sativum | - |
| seedling | - |
Glycine max | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Leishmania tropica |
| additional information | - |
overview | Glycine max |
| additional information | - |
overview | Daucus carota |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 7,8-dihydrofolate + NADPH | - |
Tetradesmus obliquus | 5,6,7,8-tetrahydrofolate + NADP+ | - |
r |  |
| 7,8-dihydrofolate + NADPH | - |
Euglena gracilis | 5,6,7,8-tetrahydrofolate + NADP+ | - |
r | |
| 7,8-dihydrofolate + NADPH | - |
Pisum sativum | 5,6,7,8-tetrahydrofolate + NADP+ | - |
r | |
| 7,8-dihydrofolate + NADPH | - |
Zea mays | 5,6,7,8-tetrahydrofolate + NADP+ | - |
r | |
| 7,8-dihydrofolate + NADPH | - |
Nicotiana tabacum | 5,6,7,8-tetrahydrofolate + NADP+ | - |
r | |
| 7,8-dihydrofolate + NADPH | - |
Glycine max | 5,6,7,8-tetrahydrofolate + NADP+ | - |
r | |
| 7,8-dihydrofolate + NADPH | - |
Crithidia fasciculata | 5,6,7,8-tetrahydrofolate + NADP+ | - |
r | |
| 7,8-dihydrofolate + NADPH | - |
Datura inoxia | 5,6,7,8-tetrahydrofolate + NADP+ | - |
r | |
| 7,8-dihydrofolate + NADPH | - |
Daucus carota | 5,6,7,8-tetrahydrofolate + NADP+ | - |
r | |
| 7,8-dihydrofolate + NADPH | - |
Oryza sativa | 5,6,7,8-tetrahydrofolate + NADP+ | - |
r | |
| 7,8-dihydrofolate + NADPH | - |
Petunia x hybrida | 5,6,7,8-tetrahydrofolate + NADP+ | - |
r | |
| 7,8-dihydrofolate + NADPH | - |
Nicotiana plumbaginifolia | 5,6,7,8-tetrahydrofolate + NADP+ | - |
r | |
| 7,8-dihydrofolate + NADPH | - |
Leishmania tropica | 5,6,7,8-tetrahydrofolate + NADP+ | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | - |
Tetradesmus obliquus |
| dimer | - |
Crithidia fasciculata |
| dimer | - |
Leishmania tropica |
| More | overview | Tetradesmus obliquus |
| More | overview | Glycine max |
| More | overview | Daucus carota |
| More | bifunctional, homodimers with DHFR and TS domains for each monomer | Crithidia fasciculata |
| More | bifunctional, homodimers with DHFR and TS domains for each monomer | Leishmania tropica |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| additional information | - |
overview | Tetradesmus obliquus |
| additional information | - |
overview | Pisum sativum |
| additional information | - |
overview | Glycine max |
| additional information | - |
overview | Daucus carota |
| additional information | - |
overview | Oryza sativa |
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