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Literature summary for 1.5.1.3 extracted from
- Tobacco budworm dihydrofolate reductase is a promising target for insecticide discovery (2000), Eur. J. Biochem., 267, 394-402.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| dithiothreitol | - |
Heliothis virescens |  |
| p-chloromercuribenzoate | - |
Heliothis virescens | |
Application
| Application | Comment | Organism |
|---|---|---|
| medicine | potentially target for the discovery of novel insecticides and anthelminthics | Heliothis virescens |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| amplification and DNA-sequencing of full length gene from DNA-library | Heliothis virescens |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| methotrexate | complete inhibition at 0.0001 mM, slow tight binding inhibitor | Heliothis virescens | |
| p-hydroxymercuribenzoate | - |
Heliothis virescens | |
| pyrimethamine | anti-malarial DHFR inhibitor | Heliothis virescens | |
| Sodium tetrathionate | thiol-blocking reagent | Heliothis virescens | |
| trimethoprim | - |
Heliothis virescens | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| soluble | - |
Drosophila melanogaster | - |
- |
| soluble | at low levels in the cytosol | Heliothis virescens | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 19000 | 21000 | DNA-sequence dtermination and SDS-PAGE | Heliothis virescens |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Drosophila melanogaster | - |
- |
- |
| Heliothis virescens | - |
i.e. Helicoverpa virescens, tobacco budworm | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Drosophila melanogaster |
- |
Heliothis virescens |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| 5,6,7,8-tetrahydrofolate + NADP+ = 7,8-dihydrofolate + NADPH + H+ | substrate and antifolate inhibitor methotrexate binding, modeling of three-dimensional structure | Heliothis virescens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| larva | - |
Heliothis virescens | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 20.8 | - |
purified enzyme | Heliothis virescens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 7,8-dihydrofolate + NADPH | strongly specific for | Drosophila melanogaster | 5,6,7,8-tetrahydrofolate + NADP+ | - |
? | |
| 7,8-dihydrofolate + NADPH | strongly specific for | Heliothis virescens | 5,6,7,8-tetrahydrofolate + NADP+ | - |
? | |
| folate + NADPH | no activity | Drosophila melanogaster | 5,6,7,8-tetrahydrofolate + NADP+ | - |
? | |
| folate + NADPH | no activity | Heliothis virescens | 5,6,7,8-tetrahydrofolate + NADP+ | - |
? | |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADPH | specific | Drosophila melanogaster | |
| NADPH | specific | Heliothis virescens | |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0000003 | - |
methotrexate | - |
Heliothis virescens | |
| 0.000021 | - |
pyrimethamine | - |
Heliothis virescens | |
| 0.00024 | - |
trimethoprim | - |
Heliothis virescens | |
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Release 2023.1 (January 2023)
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