Literature summary for 1.5.1.3 extracted from

Navarro-Martinez, M.D.; Cabezas-Herrera, J.; Garcia-Canovas, F.; Rodriguez-Lopez, J.N.
Inhibition of Stenotrophomonas maltophilia dihydrofolate reductase by methotrexate: a single slow-binding process (2007), J. Enzyme Inhib. Med. Chem., 22, 377-382.

Search for more literature for 1.5.1.3

Inhibitors

Inhibitors	Comment	Organism	Structure
methotrexate	slow-binding inhibition due to the generation of a long-lived, slowly dissociating enzyme-NADPH-inhibitor complex. One-step inhibition mechanism	Stenotrophomonas maltophilia

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0018	-	7,8-dihydrofolate	25°C, pH 7.4	Stenotrophomonas maltophilia
0.012	-	NADPH	25°C, pH 7.4	Stenotrophomonas maltophilia

Organism

Organism	UniProt	Comment	Textmining
Stenotrophomonas maltophilia	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Stenotrophomonas maltophilia

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
7,8-dihydrofolate + NADPH + H+	-	Stenotrophomonas maltophilia	5,6,7,8-tetrahydrofolate + NADP+	-	?

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
123	-	7,8-dihydrofolate	25°C, pH 7.4	Stenotrophomonas maltophilia

Cofactor

Cofactor	Comment	Organism	Structure
NADPH	-	Stenotrophomonas maltophilia

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
0.0000000289	-	methotrexate	25°C	Stenotrophomonas maltophilia

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)