Cloned (Comment) | Organism |
---|---|
a baculovirus system is established for the expression of this DHFR in Bombyx mori chrysalides. To isolate the expressed protein, whole infected pupae are homogenized | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
epicatechin gallate | - |
Homo sapiens | |
epigallocatechin gallate | - |
Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0019 | - |
dihydrofolate | pH 7.5, 25°C | Homo sapiens | |
0.0074 | - |
NADPH | pH 7.5, 25°C | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
22500 | - |
gel filtration, SDS-PAGE | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P00374 | - |
- |
Purification (Comment) | Organism |
---|---|
affinity chromatography | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dihydrofolate + NADPH + H+ | - |
Homo sapiens | tetrahydrofolate + NADP+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
DHFR | - |
Homo sapiens |
dihydrofolate reductase | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
51 | - |
NADPH | pH 7.5, 25°C, cosubstrate: dihydrofolate | Homo sapiens | |
51 | - |
dihydrofolate | pH 7.5, 25°C, cosubstrate: NADPH | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Homo sapiens |