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Literature summary for 1.5.1.30 extracted from
- Energy transfer evidence for in vitro and in vivo complexes of Vibrio harveyi flavin reductase P and luciferase (2003), Photochem. Photobiol., 77, 446-452.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene frp, functional expression of the enzyme fused to yellow fluorescence protein YFP in a Vibrio harveyi strain lacking the frp gene, subcloning and expression in Escherichia coli strains JM109 and BL21(DE3), the yellow fluorescence protein YFP is a mutant of the green fluorescence protein GFP, with mutations S65G, V68L, S72A, and T203Y | Vibrio harveyi |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.021 | - |
FMN | recombinant fusion protein, pH 7.0, 23°C | Vibrio harveyi |  |
| 0.048 | - |
NADPH | recombinant fusion protein, pH 7.0, 23°C | Vibrio harveyi | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 53783 | - |
x * 53783, recombinant fusion protein, sequence calculation | Vibrio harveyi |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Vibrio harveyi | enzyme transfers reduced riboflavin 5'-phosphate to luciferase by direct channeling in vitro and in vivo, formation of donor-acceptor enzyme complex | ? | - |
? | |
| riboflavin + NADPH | Vibrio harveyi | - |
reduced riboflavin + NADP+ | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Vibrio harveyi | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant enzyme from Escherichia coli strain BL21(DE3) to over 90% purity, recombinant fusion protein from Escherichia coli strain JM109 to over 80% purity | Vibrio harveyi |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| reduced riboflavin + NADP+ = riboflavin + NADPH + H+ | catalytic mechanism | Vibrio harveyi |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| FMN + NADPH | - |
Vibrio harveyi | FMNH2 + NADP+ | - |
? | |
| additional information | enzyme transfers reduced riboflavin 5'-phosphate to luciferase by direct channeling in vitro and in vivo, formation of donor-acceptor enzyme complex | Vibrio harveyi | ? | - |
? | |
| riboflavin + NADPH | - |
Vibrio harveyi | reduced riboflavin + NADP+ | - |
? | |
| riboflavin + NADPH | i.e. FMN | Vibrio harveyi | reduced riboflavin + NADP+ | i.e. FMNH2 | ? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 53783, recombinant fusion protein, sequence calculation | Vibrio harveyi |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| flavin reductase P | - |
Vibrio harveyi |
| FRP | - |
Vibrio harveyi |
| More | see also EC 1.5.1.29 | Vibrio harveyi |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 23 | - |
assay at | Vibrio harveyi |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
assay at | Vibrio harveyi |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADPH | - |
Vibrio harveyi | |
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