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Literature summary for 1.5.1.30 extracted from

  • Liu, G.; Zhou, J.; Lv, H.; Xiang, X.; Wang, J.; Zhou, M.; Qv, Y.
    Azoreductase from Rhodobacter sphaeroides AS1.1737 is a flavodoxin that also functions as nitroreductase and flavin mononucleotide reductase (2007), Appl. Microbiol. Biotechnol., 76, 1271-1279.
    View publication on PubMed
Search for more literature for 1.5.1.30

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Cereibacter sphaeroides

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.5
-
 NADH in the presence of FMN Cereibacter sphaeroides
14.2
-
 FMN in the presence of NADPH Cereibacter sphaeroides

Organism

Organism UniProt Comment Textmining
Cereibacter sphaeroides
-
-
-
Cereibacter sphaeroides AS1.1737
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
FMN + NADH
-
 Cereibacter sphaeroides FMNH2 + NAD+
-
 ?
FMN + NADH
-
 Cereibacter sphaeroides AS1.1737 FMNH2 + NAD+
-
 ?
FMN + NADPH
-
 Cereibacter sphaeroides FMNH2 + NADP+
-
 ?
FMN + NADPH
-
 Cereibacter sphaeroides AS1.1737 FMNH2 + NADP+
-
 ?
additional information prefers NADPH over NADH Cereibacter sphaeroides ?
-
 ?
additional information prefers NADPH over NADH Cereibacter sphaeroides AS1.1737 ?
-
 ?

Synonyms

Synonyms Comment Organism
azoreductase flavodoxin that also functions as nitroreductase and flavin mononucleotide reductase Cereibacter sphaeroides
AZRü
-
 Cereibacter sphaeroides

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
54.38
-
 FMN in the presence of NADPH Cereibacter sphaeroides

Cofactor

Cofactor Comment Organism Structure
FMN the presence of exogenously added FMN is necessary for activity Cereibacter sphaeroides
NADH prefers NADPH over NADH Cereibacter sphaeroides
NADPH preferred cofactor Cereibacter sphaeroides