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Literature summary for 1.5.1.33 extracted from
- Overexpression in Escherichia coli and purification of pteridine reductase (PTR1) from a clinical isolate of Leishmania donovani (2004), Protein Expr. Purif., 38, 228-236.
Application
| Application | Comment | Organism |
|---|---|---|
| medicine | enzyme is an important chemotherapeutic target for drugs against Leishmania | Leishmania donovani |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| overexpression of the N-terminally His-tagged enzyme in Escherichia coli strain BL21(DE3) | Leishmania donovani |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 29000 | - |
4 * 33500, recombinant enzyme, SDS-PAGE, 4 * 29000, native enzyme, SDS-PAGE | Leishmania donovani |
| 33500 | - |
4 * 33500, recombinant enzyme, SDS-PAGE, 4 * 29000, native enzyme, SDS-PAGE | Leishmania donovani |
| 134000 | - |
recombinant enzyme, gel filtration | Leishmania donovani |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Leishmania donovani | enzyme is associated with folate metabolism, responsible for salvage of pterins and reduction of folates | ? | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Leishmania donovani | Q6QDB5 | clinical isolate | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3)by nickel affinity chromatography | Leishmania donovani |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| 5,6,7,8-tetrahydrobiopterin + 2 NADP+ = biopterin + 2 NADPH + 2 H+ | conserved residue Tyr194 forms hydrogen bonds with the nucleophilic center of the substrate and stabilizes the transition state, conserved residue Lys198 lowers the pKa of the tyrosine, helps to stabilize the transition state, and may function as proton source for the tyrosine | Leishmania donovani |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | enzyme is associated with folate metabolism, responsible for salvage of pterins and reduction of folates | Leishmania donovani | ? | - |
? | |
| additional information | NADPH binds first, the enzyme-NADPH complex binds the substrate | Leishmania donovani | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | 4 * 33500, recombinant enzyme, SDS-PAGE, 4 * 29000, native enzyme, SDS-PAGE | Leishmania donovani |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| pteridine reductase 1 | - |
Leishmania donovani |
| PTR1 | - |
Leishmania donovani |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADPH | binding site is located at residues Gly13 to Arg39, recognition motif GXXXRXG | Leishmania donovani | |
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