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Literature summary for 1.5.1.33 extracted from

  • Barrack, K.L.; Tulloch, L.B.; Burke, L.A.; Fyfe, P.K.; Hunter, W.N.
    Structure of recombinant Leishmania donovani pteridine reductase reveals a disordered active site (2011), Acta Crystallogr. Sect. F, 67, 33-37.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21 (DE3) GOLD cells Leishmania donovani

Crystallization (Commentary)

Crystallization (Comment) Organism
sitting drop vapor diffusion method, using 0.1 M MES pH 6.5, 10%(v/v) dioxane and 1.6 M ammonium sulfate Leishmania donovani

Inhibitors

Inhibitors Comment Organism Structure
methotrexate
-
Leishmania donovani

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
biopterin + 2 NADPH + 2 H+ Leishmania donovani
-
5,6,7,8-tetrahydrobiopterin + 2 NADP+
-
?

Organism

Organism UniProt Comment Textmining
Leishmania donovani Q6QDB5
-
-

Purification (Commentary)

Purification (Comment) Organism
HisTrap nickel-chelating column chromatography Leishmania donovani

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
biopterin + 2 NADPH + 2 H+
-
Leishmania donovani 5,6,7,8-tetrahydrobiopterin + 2 NADP+
-
?

Subunits

Subunits Comment Organism
homotetramer x-ray crystallography Leishmania donovani

Synonyms

Synonyms Comment Organism
NADPH-dependent short-chain dehydrogenase/reductase pteridine reductase
-
Leishmania donovani
PTR1
-
Leishmania donovani

Cofactor

Cofactor Comment Organism Structure
NADPH
-
Leishmania donovani

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.000039
-
methotrexate pH and temperature not specified in the publication Leishmania donovani