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Literature summary for 1.5.1.34 extracted from

  • Williams, C.D.; Dickens, G.; Letendre, C.H.; Guroff, G.; Haines, C.; Shiota, T.
    Isolation and characterization of dihydropteridine reductase from Pseudomonas species (1976), J. Bacteriol., 127, 1197-1207.
    View publication on PubMedView publication on EuropePMC

General Stability

General Stability Organism
NADH protects against inactivation Pseudomonas sp.
NADPH protects against inactivation Pseudomonas sp.

Inhibitors

Inhibitors Comment Organism Structure
5,5'-dithiobis(2-nitrobenzoate) 0.57 mM, 98% inhibition Pseudomonas sp.
aminopterin competitive vs. 2-amino-4-hydroxy-6,7-dimethyl-quinonoid-dihydropteridine Pseudomonas sp.
CdCl2 0.1 mM, complete inactivation Pseudomonas sp.
HgCl2
-
Pseudomonas sp.
MgCl2 0.1 mM, complete inactivation Pseudomonas sp.
MnCl2 0.1 mM, complete inactivation Pseudomonas sp.
N-ethylmaleimide 5 mM, complete inhibition of reduced enzyme Pseudomonas sp.
p-chloromercuribenzoate 0.4 mM, 50% inhibition Pseudomonas sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.16
-
5,6,7,8-tetrahydrobiopterin
-
Pseudomonas sp.
0.16
-
quinonoid dihydrobiopterin
-
Pseudomonas sp.
0.34
-
quinonoid 2-amino-4-hydroxy-6,7-dimethyldihydropteridine
-
Pseudomonas sp.
0.34
-
2-Amino-4-hydroxy-6,7-dimethyl-quinonoid-dihydropteridine
-
Pseudomonas sp.
1.45
-
L-threo-neopterin
-
Pseudomonas sp.
1.45
-
quinonoid L-threo-dihydroneopterin
-
Pseudomonas sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
43500 49000 gel filtration Pseudomonas sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
quinonoid 7,8-(6H)-dihydrobiopterin + NAD(P)H Pseudomonas sp. no marked specificity for the pteridine cofactor that occurs naturally in this organism L-threo-neopterin 5,6,7,8-tetrahydrobiopterin + NAD(P)+
-
?

Organism

Organism UniProt Comment Textmining
Pseudomonas sp.
-
sp. ATCC 11299a
-

Purification (Commentary)

Purification (Comment) Organism
ATCC 11299a Pseudomonas sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
33.2
-
-
Pseudomonas sp.

Storage Stability

Storage Stability Organism
-20°C, stable for at least a year, enzyme activity in ammonium sulfate precipitate Pseudomonas sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-amino-4-hydroxy-6,7-dimethyl-quinonoid-dihydropteridine + NAD(P)H 3% activity with NADPH Pseudomonas sp. 2-amino-4-hydroxy-6,7-dimethyltetrahydropteridine + NAD(P)+
-
?
5,6,7,8-tetrahydrobiopterin + NAD+
-
Pseudomonas sp. quinonoid dihydrobiopterin + NADH + H+
-
r
L-threo-neopterin + NAD+
-
Pseudomonas sp. ?
-
r
quinoid dihydrobiopterin + NAD(P)H specific for NADH Pseudomonas sp. tetrahydrobiopterin + NAD(P)+
-
?
quinonoid 7,8-(6H)-dihydrobiopterin + NAD(P)H no marked specificity for the pteridine cofactor that occurs naturally in this organism L-threo-neopterin Pseudomonas sp. 5,6,7,8-tetrahydrobiopterin + NAD(P)+
-
?
quinonoid dihydro-L-threo-neopterin + NAD(P)H
-
Pseudomonas sp. tetrahydro-L-threo-neopterin + NAD(P)+
-
?
quinonoid dihydrobiopterin + NADH + H+
-
Pseudomonas sp. 6R-(1'R,2'R)-5,6,7,8-tetrahydrobiopterin + NAD+
-
?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
5 min, 80% loss of activity, EDTA protects Pseudomonas sp.

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
20
-
quinonoid dihydrobiopterin
-
Pseudomonas sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.2
-
-
Pseudomonas sp.

Cofactor

Cofactor Comment Organism Structure
NADH enzyme prefers NADH Pseudomonas sp.
NADPH NADH is preferred Pseudomonas sp.
NADPH inactive with NADPH, specific for NADPH Pseudomonas sp.

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.21
-
aminopterin
-
Pseudomonas sp.