General Stability | Organism |
---|---|
NADH protects against inactivation | Pseudomonas sp. |
NADPH protects against inactivation | Pseudomonas sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
5,5'-dithiobis(2-nitrobenzoate) | 0.57 mM, 98% inhibition | Pseudomonas sp. | |
aminopterin | competitive vs. 2-amino-4-hydroxy-6,7-dimethyl-quinonoid-dihydropteridine | Pseudomonas sp. | |
CdCl2 | 0.1 mM, complete inactivation | Pseudomonas sp. | |
HgCl2 | - |
Pseudomonas sp. | |
MgCl2 | 0.1 mM, complete inactivation | Pseudomonas sp. | |
MnCl2 | 0.1 mM, complete inactivation | Pseudomonas sp. | |
N-ethylmaleimide | 5 mM, complete inhibition of reduced enzyme | Pseudomonas sp. | |
p-chloromercuribenzoate | 0.4 mM, 50% inhibition | Pseudomonas sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.16 | - |
5,6,7,8-tetrahydrobiopterin | - |
Pseudomonas sp. | |
0.16 | - |
quinonoid dihydrobiopterin | - |
Pseudomonas sp. | |
0.34 | - |
quinonoid 2-amino-4-hydroxy-6,7-dimethyldihydropteridine | - |
Pseudomonas sp. | |
0.34 | - |
2-Amino-4-hydroxy-6,7-dimethyl-quinonoid-dihydropteridine | - |
Pseudomonas sp. | |
1.45 | - |
L-threo-neopterin | - |
Pseudomonas sp. | |
1.45 | - |
quinonoid L-threo-dihydroneopterin | - |
Pseudomonas sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
43500 | 49000 | gel filtration | Pseudomonas sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
quinonoid 7,8-(6H)-dihydrobiopterin + NAD(P)H | Pseudomonas sp. | no marked specificity for the pteridine cofactor that occurs naturally in this organism L-threo-neopterin | 5,6,7,8-tetrahydrobiopterin + NAD(P)+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas sp. | - |
sp. ATCC 11299a | - |
Purification (Comment) | Organism |
---|---|
ATCC 11299a | Pseudomonas sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
33.2 | - |
- |
Pseudomonas sp. |
Storage Stability | Organism |
---|---|
-20°C, stable for at least a year, enzyme activity in ammonium sulfate precipitate | Pseudomonas sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-amino-4-hydroxy-6,7-dimethyl-quinonoid-dihydropteridine + NAD(P)H | 3% activity with NADPH | Pseudomonas sp. | 2-amino-4-hydroxy-6,7-dimethyltetrahydropteridine + NAD(P)+ | - |
? | |
5,6,7,8-tetrahydrobiopterin + NAD+ | - |
Pseudomonas sp. | quinonoid dihydrobiopterin + NADH + H+ | - |
r | |
L-threo-neopterin + NAD+ | - |
Pseudomonas sp. | ? | - |
r | |
quinoid dihydrobiopterin + NAD(P)H | specific for NADH | Pseudomonas sp. | tetrahydrobiopterin + NAD(P)+ | - |
? | |
quinonoid 7,8-(6H)-dihydrobiopterin + NAD(P)H | no marked specificity for the pteridine cofactor that occurs naturally in this organism L-threo-neopterin | Pseudomonas sp. | 5,6,7,8-tetrahydrobiopterin + NAD(P)+ | - |
? | |
quinonoid dihydro-L-threo-neopterin + NAD(P)H | - |
Pseudomonas sp. | tetrahydro-L-threo-neopterin + NAD(P)+ | - |
? | |
quinonoid dihydrobiopterin + NADH + H+ | - |
Pseudomonas sp. | 6R-(1'R,2'R)-5,6,7,8-tetrahydrobiopterin + NAD+ | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
5 min, 80% loss of activity, EDTA protects | Pseudomonas sp. |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
20 | - |
quinonoid dihydrobiopterin | - |
Pseudomonas sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.2 | - |
- |
Pseudomonas sp. |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | enzyme prefers NADH | Pseudomonas sp. | |
NADPH | NADH is preferred | Pseudomonas sp. | |
NADPH | inactive with NADPH, specific for NADPH | Pseudomonas sp. |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.21 | - |
aminopterin | - |
Pseudomonas sp. |