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Literature summary for 1.5.1.34 extracted from
- A simple procedure for purification of NADH-specific dihydropteridine reductase from mammalian liver (1982), J. Biochem., 92, 1033-1040.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00085 | - |
NADH | - |
Rattus norvegicus |  |
| 0.00085 | - |
NADH | NADH-specific liver enzyme | Rattus norvegicus | |
| 0.0034 | - |
quinonoiddihydro-6-methylpterin | - |
Rattus norvegicus | |
| 0.076 | - |
NADPH | NADH-specific liver enzyme | Rattus norvegicus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 25000 | - |
2 * 25000, NADH-specific liver enzyme, SDS-PAGE | Rattus norvegicus |
| 49000 | - |
- |
Bos taurus |
| 49000 | - |
NADH-specific liver enzyme, gel filtration | Rattus norvegicus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| quinonoid 7,8-(6H)-dihydrobiopterin + NAD(P)H | Homo sapiens | - |
5,6,7,8-tetrahydrobiopterin + NAD(P)+ | - |
? | |
| quinonoid 7,8-(6H)-dihydrobiopterin + NAD(P)H | Bos taurus | - |
5,6,7,8-tetrahydrobiopterin + NAD(P)+ | - |
? | |
| quinonoid 7,8-(6H)-dihydrobiopterin + NAD(P)H | Rattus norvegicus | essential component of catecholamine biosynthetic metabolism | 5,6,7,8-tetrahydrobiopterin + NAD(P)+ | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bos taurus | - |
- |
- |
| Homo sapiens | - |
- |
- |
| Rattus norvegicus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| simple two step procedure i.e. affinity chromatography on Matrex gel blue A and hydrophobic chromatography on phenyl-Sepharose | Homo sapiens |
| simple two step procedure i.e. affinity chromatography on Matrex gel blue A and hydrophobic chromatography on phenyl-Sepharose | Rattus norvegicus |
| simple two step procedure i.e. affinity chromatography on Matrex gel blue A and hydrophobic chromatography on phenyl-Sepharose | Bos taurus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | - |
Homo sapiens | - |
| liver | - |
Rattus norvegicus | - |
| liver | - |
Bos taurus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 20.2 | - |
NADH-specific liver enzyme | Homo sapiens |
| 24 | - |
NADH-specific liver enzyme | Rattus norvegicus |
| 26.2 | - |
NADH-specific liver enzyme | Bos taurus |
| 64.2 | - |
- |
Rattus norvegicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| quinonoid 7,8-(6H)-dihydrobiopterin + NAD(P)H | - |
Homo sapiens | 5,6,7,8-tetrahydrobiopterin + NAD(P)+ | - |
? | |
| quinonoid 7,8-(6H)-dihydrobiopterin + NAD(P)H | - |
Bos taurus | 5,6,7,8-tetrahydrobiopterin + NAD(P)+ | - |
? | |
| quinonoid 7,8-(6H)-dihydrobiopterin + NAD(P)H | essential component of catecholamine biosynthetic metabolism | Rattus norvegicus | 5,6,7,8-tetrahydrobiopterin + NAD(P)+ | - |
? | |
| quinonoiddihydro-6-methylpterin + NADH + H+ | - |
Rattus norvegicus | ? | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | - |
Homo sapiens |
| dimer | - |
Bos taurus |
| dimer | 2 * 25000, NADH-specific liver enzyme, SDS-PAGE | Rattus norvegicus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.6 | - |
NADH-specific liver enzyme | Rattus norvegicus |
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