Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Mycobacterium goodii |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ag+ | strong inhibition at 1 mM Ag+ | Mycobacterium goodii | |
Cu2+ | strong inhibition at 1 mM Cu2+ | Mycobacterium goodii | |
Hg2+ | strong inhibition at 1 mM Hg2+ | Mycobacterium goodii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0066 | - |
FMN | purified enzyme, at pH 7.0 and 55°C | Mycobacterium goodii | |
0.0779 | - |
NADH | purified enzyme, at pH 7.0 and 55°C | Mycobacterium goodii |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Cr3+ | slight activation by 1 mM Cr3+ | Mycobacterium goodii | |
additional information | the enzyme is not susceptible to Ca2+, Mg2+, Mn2+ and Co2+ | Mycobacterium goodii | |
Zn2+ | slight activation by 1 mM Zn2+ | Mycobacterium goodii |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
16989 | - |
2 * 16989, calculated from amino acid sequence | Mycobacterium goodii |
17000 | - |
2 * 17000, SDS-PAGE | Mycobacterium goodii |
39000 | - |
gel filtration | Mycobacterium goodii |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
FMN + NADH + H+ | Mycobacterium goodii | - |
FMNH2 + NAD+ | - |
? | |
FMN + NADH + H+ | Mycobacterium goodii X7B | - |
FMNH2 + NAD+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycobacterium goodii | - |
- |
- |
Mycobacterium goodii X7B | - |
- |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation, butyl-Sepharose column chromatography, Source 30Q column chromatography, FMN-Sepharose 6B affinity column chromatography, and Superdex 75 gel filtration | Mycobacterium goodii |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.6 | - |
using riboflavin and NADPH as substrates, at pH 7.0 and 55°C | Mycobacterium goodii |
0.8 | - |
using FAD and NADPH as substrates, at pH 7.0 and 55°C | Mycobacterium goodii |
1.3 | - |
using FMN and NADPH as substrates, at pH 7.0 and 55°C | Mycobacterium goodii |
36 | - |
using riboflavin and NADH as substrates, at pH 7.0 and 55°C | Mycobacterium goodii |
189 | - |
using FAD and NADH as substrates, at pH 7.0 and 55°C | Mycobacterium goodii |
617 | - |
using FMN and NADH as substrates, at pH 7.0 and 55°C | Mycobacterium goodii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
FAD + NADH + H+ | the relative activity with FAD is approximately 31% of that of FMN as the acceptor | Mycobacterium goodii | FADH2 + NAD+ | - |
? | |
FAD + NADH + H+ | the relative activity with FAD is approximately 31% of that of FMN as the acceptor | Mycobacterium goodii X7B | FADH2 + NAD+ | - |
? | |
FMN + NADH + H+ | - |
Mycobacterium goodii | FMNH2 + NAD+ | - |
? | |
FMN + NADH + H+ | - |
Mycobacterium goodii X7B | FMNH2 + NAD+ | - |
? | |
additional information | less than 0.3% of enzyme activity remains when NADPH is used as the electron donor instead of NADH | Mycobacterium goodii | ? | - |
? | |
additional information | less than 0.3% of enzyme activity remains when NADPH is used as the electron donor instead of NADH | Mycobacterium goodii X7B | ? | - |
? | |
riboflavin + NADH + H+ | the relative activity with riboflavin is approximately 5.8% of that of FMN as the acceptor | Mycobacterium goodii | reduced riboflavin + NAD+ | - |
? | |
riboflavin + NADH + H+ | the relative activity with riboflavin is approximately 5.8% of that of FMN as the acceptor | Mycobacterium goodii X7B | reduced riboflavin + NAD+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 17000, SDS-PAGE | Mycobacterium goodii |
homodimer | 2 * 16989, calculated from amino acid sequence | Mycobacterium goodii |
Synonyms | Comment | Organism |
---|---|---|
DszD | - |
Mycobacterium goodii |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
- |
Mycobacterium goodii |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | 60 | enzyme activity is stable at temperatures below 45°C. After incubation at 60°C for 30 min, more than 90% of the activity is lost | Mycobacterium goodii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.6 | - |
- |
Mycobacterium goodii |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FMN | each subunit binds one FMN as cofactor | Mycobacterium goodii | |
NADH | - |
Mycobacterium goodii |