Literature summary for 1.5.1.36 extracted from

Wang, L.; Ma, X.; Ruan, H.; Chen, Y.; Gao, L.; Lei, T.; Li, Y.; Gui, L.; Guo, L.; Xia, T.; Wang, Y.
Optimization of the biosynthesis of B-ring ortho-hydroxylated flavonoids using the 4-hydroxyphenylacetate 3-hydroxylase complex (HpaBC) of Escherichia coli (2021), Molecules, 26, 2919 .

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Application

Application	Comment	Organism
synthesis	in vivo production of ortho-hydroxylated flavonoids by recombinant Escherichia coli. When HpaC is linked with an S-Tag on the C terminus, the enzyme activity is significantly affected. The optimal culture conditions are a substrate concentration of 80 mg/l, an induction temperature of 28°C, an M9 medium, and a substrate delay time of 6 h after IPTG induction. The efficiency of eriodictyol conversion from recombinant strains fed naringin is up to 57.67. Highest conversion efficiencies for production of catechin and caffeate are 35.2 % and 32.93%, respectively	Escherichia coli

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Escherichia coli

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	A0A140NG67	-	-
Escherichia coli BL21-DE3	A0A140NG67	-	-

Subunits

Subunits	Comment	Organism
?	x * 58500, SDS-PAGE, recombinant protein	Escherichia coli

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Release 2023.1 (January 2023)