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Literature summary for 1.5.1.40 extracted from

  • Elias, D.A.; Juck, D.F.; Berry, K.A.; Sparling, R.
    Purification of the NADP+:F420 oxidoreductase of Methanosphaera stadtmanae (2000), Can. J. Microbiol., 46, 998-1003.
    View publication on PubMed
Search for more literature for 1.5.1.40

General Stability

General Stability Organism
neither high salt or protease inhibitors were effective in stabilizing the activity of the partially purified enzyme Methanosphaera stadtmanae
precipitation with (NH4)2SO4 reduces the stability of the enzyme Methanosphaera stadtmanae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0077
-
 reduced coenzyme F420 pH and temperature not specified in the publication Methanosphaera stadtmanae
0.0625
-
 coenzyme F420 pH and temperature not specified in the publication Methanosphaera stadtmanae
0.142
-
 NADPH pH and temperature not specified in the publication Methanosphaera stadtmanae
0.37
-
 NADP+ pH and temperature not specified in the publication Methanosphaera stadtmanae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
45000
-
 1 * 60000 (alpha) + 1 * 50000 (beta) + 1 * 45000 (gamma), SDS-PAGE Methanosphaera stadtmanae
50000
-
 1 * 60000 (alpha) + 1 * 50000 (beta) + 1 * 45000 (gamma), SDS-PAGE Methanosphaera stadtmanae
60000
-
 1 * 60000 (alpha) + 1 * 50000 (beta) + 1 * 45000 (gamma), SDS-PAGE Methanosphaera stadtmanae
148000
-
 gel filtration, native PAGE Methanosphaera stadtmanae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
reduced coenzyme F420 + NADP+ Methanosphaera stadtmanae
-
 coenzyme F420 + NADPH + H+
-
 r
reduced coenzyme F420 + NADP+ Methanosphaera stadtmanae DSM 3091
-
 coenzyme F420 + NADPH + H+
-
 r

Organism

Organism UniProt Comment Textmining
Methanosphaera stadtmanae
-
-
-
Methanosphaera stadtmanae DSM 3091
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Methanosphaera stadtmanae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
reduced coenzyme F420 + NADP+
-
 Methanosphaera stadtmanae coenzyme F420 + NADPH + H+
-
 r
reduced coenzyme F420 + NADP+
-
 Methanosphaera stadtmanae DSM 3091 coenzyme F420 + NADPH + H+
-
 r

Subunits

Subunits Comment Organism
trimer 1 * 60000 (alpha) + 1 * 50000 (beta) + 1 * 45000 (gamma), SDS-PAGE Methanosphaera stadtmanae

Synonyms

Synonyms Comment Organism
NADP+:F420 oxidoreductase
-
 Methanosphaera stadtmanae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
 reduction of coenzyme F420 Methanosphaera stadtmanae
8.5
-
 reduction of NADP+ Methanosphaera stadtmanae