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Literature summary for 1.5.1.42 extracted from
- Identification of NADH-specific and NADPH-specific FMN reductases in Beneckea harveyi (1975), Eur. J. Biochem., 57, 461-467.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0011 | - |
FMN | pH 6.8, 23°C | Vibrio harveyi |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 19000 | - |
gel filtration | Vibrio harveyi |
| 31000 | - |
sucrose density gradient centrifugation | Vibrio harveyi |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| FMN + NADH + H+ | Vibrio harveyi | - |
FMNH2 + NAD+ | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Vibrio harveyi | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Vibrio harveyi |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 2.05 | - |
pH 6.8, 23°C | Vibrio harveyi |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, considerable loss of activity of reductase preparations | Vibrio harveyi |
| 4°C, considerable loss of activity of reductase preparations | Vibrio harveyi |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| FMN + NADH + H+ | - |
Vibrio harveyi | FMNH2 + NAD+ | - |
? | |
| FMN + NADH + H+ | the affinity of the NADH-specific reductase is about 60 times greater for NADH than for NADPH | Vibrio harveyi | FMNH2 + NAD+ | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 23 | - |
assay at | Vibrio harveyi |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 55 | - |
5 min, 60% loss of activity | Vibrio harveyi |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.8 | - |
assay at | Vibrio harveyi |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADH | the affinity of the NADH-specific reductase is about 60 times greater for NADH than for NADPH | Vibrio harveyi | |
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Release 2023.1 (January 2023)
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