Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,10-phenanthroline | 0.005 M, 52% inhibition | Vibrio harveyi | |
5,5'-dithiobis(2-nitrobenzoic acid) | 0.001 M, 38% inhibition | Vibrio harveyi | |
AMP | 0.01 M, 36% inhibition | Vibrio harveyi | |
additional information | no inhibition by: 0.000325 M dicoumarol, 10 mM KCN, 1% Triton X-100 | Vibrio harveyi | |
N-ethylmaleimide | 0.001 M, 38% inhibition | Vibrio harveyi | |
p-hydroxymercuribenzoate | 0.001 M, 49% inhibition | Vibrio harveyi | |
rotenone | 0.0004 M, 20% inhibition | Vibrio harveyi |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.001 | - |
FMN | pH 5.6, 23°C, spectrometric assay | Vibrio harveyi | |
0.001 | - |
FMN | pH 8.5, 23°C, spectrometric assay | Vibrio harveyi | |
0.012 | - |
NADH | pH 8.5, 23°C, spectrometric assay | Vibrio harveyi | |
0.047 | - |
NADH | pH 5.6, 23°C, spectrometric assay | Vibrio harveyi | |
0.0475 | - |
NADH | pH 7.0, 23°C | Vibrio harveyi |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
30000 | - |
gel filtration | Vibrio harveyi |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
FMN + NADPH + H+ | Vibrio harveyi | - |
FMNH2 + NADP+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Vibrio harveyi | - |
- |
- |
Vibrio harveyi No. 392 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Vibrio harveyi |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
FMNH2 + NAD+ = FMN + NADH + H+ | the enzyme exhibits single displacement kinetics | Vibrio harveyi |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
31 | - |
pH 7.0, 23°C | Vibrio harveyi |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
FAD + NADH + H+ | activity is 75% of that with FMN | Vibrio harveyi | FADH2 + NAD+ | - |
? | |
FMN + NADH + H+ | - |
Vibrio harveyi | FMNH2 + NAD+ | - |
? | |
FMN + NADH + H+ | the NADH specific FMN reductase does dehydrogenate NADPH with a maximal velocity one-tenth of that for NADH | Vibrio harveyi | FMNH2 + NAD+ | - |
? | |
FMN + NADH + H+ | - |
Vibrio harveyi No. 392 | FMNH2 + NAD+ | - |
? | |
FMN + NADPH + H+ | - |
Vibrio harveyi | FMNH2 + NADP+ | - |
? | |
FMN + NADPH + H+ | the NADH specific FMN reductase does dehydrogenate NADPH with a maximal velocity one-tenth of that for NADH | Vibrio harveyi | FMNH2 + NADP+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
NADH specific FMN reductase | - |
Vibrio harveyi |
NADH:FMN oxidoreductase | - |
Vibrio harveyi |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
23 | - |
assay at | Vibrio harveyi |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
15.5 | - |
NADPH | pH 7.0, 23°C | Vibrio harveyi |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.5 | - |
- |
Vibrio harveyi |
7 | - |
assay at | Vibrio harveyi |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 8.6 | the pH curve is bimodal, with maximal activity exhibited at pH 8.6, a minimum at pH 6.0. and a second maximum at pH 5.0 | Vibrio harveyi |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | the NADH specific FMN reductase does does dehydrogenate NADPH with a maximal velocity one-tenth of that for NADH | Vibrio harveyi |