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Literature summary for 1.5.1.42 extracted from

  • Jablonski, E.; DeLuca, M.
    Purification and properties of the NADH and NADPH specific FMN oxidoreductases from Beneckea harveyi (1977), Biochemistry, 16, 2932-2936.
    View publication on PubMed
Search for more literature for 1.5.1.42

Inhibitors

Inhibitors Comment Organism Structure
1,10-phenanthroline 0.005 M, 52% inhibition Vibrio harveyi
5,5'-dithiobis(2-nitrobenzoic acid) 0.001 M, 38% inhibition Vibrio harveyi
AMP 0.01 M, 36% inhibition Vibrio harveyi
additional information no inhibition by: 0.000325 M dicoumarol, 10 mM KCN, 1% Triton X-100 Vibrio harveyi
N-ethylmaleimide 0.001 M, 38% inhibition Vibrio harveyi
p-hydroxymercuribenzoate 0.001 M, 49% inhibition Vibrio harveyi
rotenone 0.0004 M, 20% inhibition Vibrio harveyi

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.001
-
 FMN pH 5.6, 23°C, spectrometric assay Vibrio harveyi
0.001
-
 FMN pH 8.5, 23°C, spectrometric assay Vibrio harveyi
0.012
-
 NADH pH 8.5, 23°C, spectrometric assay Vibrio harveyi
0.047
-
 NADH pH 5.6, 23°C, spectrometric assay Vibrio harveyi
0.0475
-
 NADH pH 7.0, 23°C Vibrio harveyi

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
30000
-
 gel filtration Vibrio harveyi

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
FMN + NADPH + H+ Vibrio harveyi
-
 FMNH2 + NADP+
-
 ?

Organism

Organism UniProt Comment Textmining
Vibrio harveyi
-
-
-
Vibrio harveyi No. 392
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Vibrio harveyi

Reaction

Reaction Comment Organism Reaction ID
FMNH2 + NAD+ = FMN + NADH + H+ the enzyme exhibits single displacement kinetics Vibrio harveyi
a

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
31
-
 pH 7.0, 23°C Vibrio harveyi

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
FAD + NADH + H+ activity is 75% of that with FMN Vibrio harveyi FADH2 + NAD+
-
 ?
FMN + NADH + H+
-
 Vibrio harveyi FMNH2 + NAD+
-
 ?
FMN + NADH + H+ the NADH specific FMN reductase does dehydrogenate NADPH with a maximal velocity one-tenth of that for NADH Vibrio harveyi FMNH2 + NAD+
-
 ?
FMN + NADH + H+
-
 Vibrio harveyi No. 392 FMNH2 + NAD+
-
 ?
FMN + NADPH + H+
-
 Vibrio harveyi FMNH2 + NADP+
-
 ?
FMN + NADPH + H+ the NADH specific FMN reductase does dehydrogenate NADPH with a maximal velocity one-tenth of that for NADH Vibrio harveyi FMNH2 + NADP+
-
 ?

Synonyms

Synonyms Comment Organism
NADH specific FMN reductase
-
 Vibrio harveyi
NADH:FMN oxidoreductase
-
 Vibrio harveyi

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
23
-
 assay at Vibrio harveyi

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
15.5
-
 NADPH pH 7.0, 23°C Vibrio harveyi

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5
-
-
 Vibrio harveyi
7
-
 assay at Vibrio harveyi

pH Range

pH Minimum pH Maximum Comment Organism
5 8.6 the pH curve is bimodal, with maximal activity exhibited at pH 8.6, a minimum at pH 6.0. and a second maximum at pH 5.0 Vibrio harveyi

Cofactor

Cofactor Comment Organism Structure
NADPH the NADH specific FMN reductase does does dehydrogenate NADPH with a maximal velocity one-tenth of that for NADH Vibrio harveyi