Application | Comment | Organism |
---|---|---|
analysis | Photobacterium leiognathi Lux G might contribute to the development of a NADH detection kit | Photobacterium leiognathi |
Cloned (Comment) | Organism |
---|---|
gene luxG, phylogenetic analysis, cloning and recombinant overexpression of His-tagged enzyme in Escherichia coli strain BL21(DE3) | Photobacterium leiognathi |
Protein Variants | Comment | Organism |
---|---|---|
additional information | establishment of a coupled pure enzyme bioluminescent system and usage for NADH detection | Photobacterium leiognathi |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
FMN + NADH + H+ | Photobacterium leiognathi | - |
FMNH2 + NAD+ | - |
? | |
FMN + NADH + H+ | Photobacterium leiognathi YL | - |
FMNH2 + NAD+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Photobacterium leiognathi | P29237 | - |
- |
Photobacterium leiognathi YL | P29237 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Photobacterium leiognathi |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
FMN + NADH + H+ | - |
Photobacterium leiognathi | FMNH2 + NAD+ | - |
? | |
FMN + NADH + H+ | - |
Photobacterium leiognathi YL | FMNH2 + NAD+ | - |
? | |
additional information | establishment of a coupled pure enzyme bioluminescent system and usage for quantitative detection, method evaluation | Photobacterium leiognathi | ? | - |
- |
|
additional information | establishment of a coupled pure enzyme bioluminescent system and usage for quantitative detection, method evaluation | Photobacterium leiognathi YL | ? | - |
- |
Subunits | Comment | Organism |
---|---|---|
? | x * 26000, recombinant His-tagged enzyme, SDS-PAGE | Photobacterium leiognathi |
Synonyms | Comment | Organism |
---|---|---|
flavin reductase | - |
Photobacterium leiognathi |
LuxG | - |
Photobacterium leiognathi |
NADH:flavin oxidoreductase | - |
Photobacterium leiognathi |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
recombinant His-tagged enzyme | Photobacterium leiognathi |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
recombinant His-tagged enzyme | Photobacterium leiognathi |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | - |
Photobacterium leiognathi |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Photobacterium leiognathi | recombinant His-tagged enzyme, isoelectric focusing | - |
6 |
General Information | Comment | Organism |
---|---|---|
evolution | phylogenetic analysis demonstrats that Photobacterium leiognathi strain YL LuxG has a rather distant evolutionary relationship with Frase I of Aliivibrio fischeri and Frp of Vibrio harveyi, but a close evolutionary relationship with Fre from Escherichia coli, which are all enzymes related to oxidoreductases. Changes in the functionally conserved sites contribute to the functional divergence of LuxG and Fre. Bioinformatics analysis of LuxG sequences in bacteria, overview. Structure comparisons | Photobacterium leiognathi |
physiological function | LuxG supplies reduced flavin mononucleotide (FMN) for bacterial luminescence by catalyzing the oxidation of nicotinamide adenine dinucleotide hydrogen (NADH) | Photobacterium leiognathi |