Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,10-phenanthroline | 1 mM, 78% inhibition | Streptomyces sp. | |
2,2'-bipyridyl | 1 mM, 75% inhibition | Streptomyces sp. | |
4-chloromercuribenzoate | 0.1 mM, 97% inhibition | Streptomyces sp. | |
8-hydroxyquinoline | 1 mM, 94% inhibition | Streptomyces sp. | |
AgNO3 | 1 mM, 97% inhibition | Streptomyces sp. | |
CuCl2 | 1 mM, 41% inhibition | Streptomyces sp. | |
Cuprizone | 0.1 mM, 87% inhibition | Streptomyces sp. | |
HgCl2 | 1 mM, 75% inhibition | Streptomyces sp. | |
additional information | no inhibition: EDTA, Tiron, CaCl2, CdCl2, CoCl2, FeCl3, LiOH, MgCl2, NiCl2, ZnSO4, semicarbazide, diethylamine cysteamine, 3-(aminomethyl)pyridine, iodoacetate, (R)-(+)-cycloserine, diethyldithiocarbamiate, N-ethylmaleimide | Streptomyces sp. | |
PMSF | 0.1 mM, 93% inhibition | Streptomyces sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0037 | - |
NADPH | 30°C, pH 7.0 | Streptomyces sp. | |
3.52 | - |
2-methyl-1-pyrroline | 30°C, pH 7.0 | Streptomyces sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
32000 | - |
2 * 32000, SDS-PAGE | Streptomyces sp. |
65000 | - |
gel filtration | Streptomyces sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-methyl-1-pyrroline + NADPH + H+ | Streptomyces sp. | - |
(R)-2-methylpyrrolidine + NADP+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces sp. | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Streptomyces sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.00032 | - |
30°C, pH 7.0, oxidation of 2-methyl-1-pyrroline | Streptomyces sp. |
10.2 | - |
30°C, pH 7.0, reduction of 2-methyl-1-pyrroline | Streptomyces sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-methyl-1-pyrroline + NADPH + H+ | - |
Streptomyces sp. | (R)-2-methylpyrrolidine + NADP+ | - |
r | |
2-methyl-1-pyrroline + NADPH + H+ | the enzyme is very specific for 2-methyl-1-pyrroline as substrate. The purified enzyme catalyzes the formation of (R)-2-methylpyrrolidine with 99% enantiomeric excess. The enzyme shows also oxidation activity for (R)-2-methyl-1-pyrroline under alkaline pH (10-11.5) conditions. No activity with (S)-2-methylpyrrolidine. No activity with NADH | Streptomyces sp. | (R)-2-methylpyrrolidine + NADP+ | - |
r | |
additional information | no activity with DELTA1-pyrroline-2-carboxylate, methyl-DELTA1-pyrroline-2-carboxylate, dihydrofolate, DELTA1-piperidine-2-carboxylate, 1-methyl-3,4-dihydroisoquinoline, 1-methyl-6,7-dimethoxy-3,4-dihydroisoquinoline, N-benzylidenebenzylamine, (E)-N-(1-phenylethylidene)benzeneamine ketone, 4-hydroxy-2-butanone, ethyl-3-oxobutanoate, ethyl 4-chloro-3-oxobutanoate, 3-2,2,6-trimethyl-2-cyclohexen-1,4-dione, 2-methylfuran, 2-methylimidazole, 2-methylthiazole, 2-methylpyrimidine, 2-methylpyrazine, 1,4,5,6-tetrahydropyrimidine | Streptomyces sp. | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 32000, SDS-PAGE | Streptomyces sp. |
Synonyms | Comment | Organism |
---|---|---|
(R)-imine reductase | - |
Streptomyces sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Streptomyces sp. |
50 | - |
- |
Streptomyces sp. |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | - |
stable below, 30 min | Streptomyces sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
reduction of 2-methyl-1-pyrroline | Streptomyces sp. |
7 | - |
assay at | Streptomyces sp. |
11 | - |
oxidation of (R)-2-methylpyrrolidine | Streptomyces sp. |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.2 | 8.7 | pH 6.2: about 35% of maximal activity, pH 8.7: about 55% of maximal activity, reduction of 2-methyl-1-pyrroline | Streptomyces sp. |
10.2 | 11.7 | pH 6.2: about 60% of maximal activity, pH 8.7: about 60% of maximal activity, oxidation of (R)-2-methylpyrrolidine | Streptomyces sp. |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | NADPH-dependent enzyme. No activity with NADH | Streptomyces sp. |