KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.79 | - |
DELTA1-pyrroline-2-carboxylate | pH 6.5, 30°C, cosubstrate: NADH | Colwellia psychrerythraea | |
5.9 | - |
DELTA1-pyrroline-2-carboxylate | pH 6.5, 30°C, cosubstrate: NADPH | Colwellia psychrerythraea | |
18.3 | - |
L-proline | pH 10.5, 30°C, cosubstrate: NADP+ | Colwellia psychrerythraea | |
18.8 | - |
L-proline | pH 10.5, 30°C, cosubstrate: NAD+ | Colwellia psychrerythraea |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
DELTA1-pyrroline-2-carboxylate + NADH + H+ | Colwellia psychrerythraea | the enzyme is part of the trans-3-hydroxy-L-proline pathway | L-proline + NAD+ | - |
? | |
DELTA1-pyrroline-2-carboxylate + NADPH + H+ | Colwellia psychrerythraea | the enzyme is part of the trans-3-hydroxy-L-proline pathway | L-proline + NADP+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Colwellia psychrerythraea | Q485S0 | - |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.00451 | - |
L-proline, pH 10.5, 30°C, cosubstrate: NAD+ | Colwellia psychrerythraea |
0.63 | - |
L-proline, pH 10.5, 30°C, cosubstrate: NADP+ | Colwellia psychrerythraea |
4.76 | - |
DELTA1-pyrroline-2-carboxylate + NADH, pH 6.5, 30°C | Colwellia psychrerythraea |
30.2 | - |
DELTA1-pyrroline-2-carboxylate + NADPH, pH 6.5, 30°C | Colwellia psychrerythraea |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
DELTA1-pyrroline-2-carboxylate + NADH + H+ | the enzyme is part of the trans-3-hydroxy-L-proline pathway | Colwellia psychrerythraea | L-proline + NAD+ | - |
? | |
DELTA1-pyrroline-2-carboxylate + NADH + H+ | preference of the reaction equilibrium to the direction toward NADPH dependent reduction. The kcat/Km value for DELTA1-pyrroline-2-carboxylate in the presence of NADH is 5.6fold lower than that in the presence of NADPH, mainly caused by the 12fold lower kcat value | Colwellia psychrerythraea | L-proline + NAD+ | - |
? | |
DELTA1-pyrroline-2-carboxylate + NADPH + H+ | the enzyme is part of the trans-3-hydroxy-L-proline pathway | Colwellia psychrerythraea | L-proline + NADP+ | - |
? | |
DELTA1-pyrroline-2-carboxylate + NADPH + H+ | preference of the reaction equilibrium to the direction toward NADPH dependent reduction. The preferred cofactor is NADPH, lower activity with NADH. The kcat/Km value for DELTA1-pyrroline-2-carboxylate in the presence of NADH is 5.6fold lower than that in the presence of NADPH, mainly caused by the 12fold lower kcat value | Colwellia psychrerythraea | L-proline + NADP+ | - |
? | |
L-proline + NAD+ | preference of the reaction equilibrium to the direction toward NADH dependent reduction. In the oxidization of L-proline the ratio of NADP+ to NAD+ in kcat/Km value is about 150:1 | Colwellia psychrerythraea | DELTA1-pyrroline-2-carboxylate + NADH + H+ | - |
r | |
L-proline + NADP+ | preference of the reaction equilibrium to the direction toward NADH dependent reduction. In the oxidization of L-proline the ratio of NADP+ to NAD+ in kcat/Km value is about 150:1 | Colwellia psychrerythraea | DELTA1-pyrroline-2-carboxylate + NADPH + H+ | - |
r | |
additional information | no significant activity with DELTA1-piperideine-2-carboxylate or L-pipecolate. The kcat/Km values for DELTA1-piperideine-2-carboxylate and L-pipecolate in the presence of NADP+(H) are 111fold and 223fold lower than those for DELTA1-pyrroline-2-carboxylate and L-proline, mainly caused by 90fold and 51fold lower kcat values, respectively | Colwellia psychrerythraea | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
LhpH | - |
Colwellia psychrerythraea |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Colwellia psychrerythraea |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0069 | - |
L-proline | pH 10.5, 30°C, cosubstrate: NAD+ | Colwellia psychrerythraea | |
1.01 | - |
L-proline | pH 10.5, 30°C, cosubstrate: NADP+ | Colwellia psychrerythraea | |
10.35 | - |
DELTA1-pyrroline-2-carboxylate | pH 6.5, 30°C, cosubstrate: NADH | Colwellia psychrerythraea | |
124.5 | - |
DELTA1-pyrroline-2-carboxylate | pH 6.5, 30°C, cosubstrate: NADPH | Colwellia psychrerythraea |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
assay at, reduction of DELTA1-pyrroline-2-carboxylate | Colwellia psychrerythraea |
10.5 | - |
assay at, oxidation of L-proline | Colwellia psychrerythraea |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | in the oxidization of L-proline the ratio of NADP+ to NAD+ in kcat/Km value is about 150:1 | Colwellia psychrerythraea | |
NADH | the preferred cofactor is NADPH, lower activity with NADH. The kcat/Km value for DELTA1-pyrroline-2-carboxylate in the presence of NADH is 5.6fold lower than that in the presence of NADPH, mainly caused by the 12fold lower kcat value | Colwellia psychrerythraea | |
NADP+ | in the oxidization of L-proline the ratio of NADP+ to NAD+ in kcat/Km value is about 150:1 | Colwellia psychrerythraea | |
NADPH | the preferred cofactor is NADPH, lower activity with NADH. The kcat/Km value for DELTA1-pyrroline-2-carboxylate in the presence of NADH is 5.6fold lower than that in the presence of NADPH, mainly caused by the 12fold lower kcat value | Colwellia psychrerythraea |
General Information | Comment | Organism |
---|---|---|
metabolism | the enzyme is part of the trans-3-hydroxy-L-proline pathway | Colwellia psychrerythraea |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.000367 | - |
L-proline | pH 10.5, 30°C, cosubstrate: NAD+ | Colwellia psychrerythraea | |
0.05519 | - |
L-proline | pH 10.5, 30°C, cosubstrate: NADP+ | Colwellia psychrerythraea | |
3.7 | - |
DELTA1-pyrroline-2-carboxylate | pH 6.5, 30°C, cosubstrate: NADH | Colwellia psychrerythraea | |
21.1 | - |
DELTA1-pyrroline-2-carboxylate | pH 6.5, 30°C, cosubstrate: NADPH | Colwellia psychrerythraea |