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Literature summary for 1.5.1.5 extracted from
- Purification and characterization of the methylene tetrahydromethanopterin dehydrogenase MtdB and the methylene tetrahydrofolate dehydrogenase FolD from Hyphomicrobium zavarzinii ZV580 (2002), Arch. Microbiol., 177, 299-303.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.024 | - |
5,10-methylenetetrahydrofolate | bifunctional methylenetetrahydrofolate dehydrogenase-cyclohydrolase enzyme | Hyphomicrobium zavarzinii |  |
| 0.13 | - |
NADP+ | bifunctional methylenetetrahydrofolate dehydrogenase-cyclohydrolase enzyme | Hyphomicrobium zavarzinii | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 31000 | - |
x * 31000, SDS-PAGE | Hyphomicrobium zavarzinii |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5,10-methylenetetrahydrofolate + NADP+ | Hyphomicrobium zavarzinii | - |
5,10-methenyltetrahydrofolate + NADPH + H+ | - |
? | |
| 5,10-methylenetetrahydrofolate + NADP+ | Hyphomicrobium zavarzinii ZV580 | - |
5,10-methenyltetrahydrofolate + NADPH + H+ | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Hyphomicrobium zavarzinii | - |
ZV580 | - |
| Hyphomicrobium zavarzinii ZV580 | - |
ZV580 | - |
| no activity in Methylobacterium extorquens | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| bifunctional methylenetetrahydrofolate dehydrogenase-cyclohydrolase | Hyphomicrobium zavarzinii |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Hyphomicrobium zavarzinii |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5,10-methylenetetrahydrofolate + NADP+ | - |
Hyphomicrobium zavarzinii | 5,10-methenyltetrahydrofolate + NADPH + H+ | - |
? | |
| 5,10-methylenetetrahydrofolate + NADP+ | - |
Hyphomicrobium zavarzinii ZV580 | 5,10-methenyltetrahydrofolate + NADPH + H+ | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 31000, SDS-PAGE | Hyphomicrobium zavarzinii |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | 6.5 | - |
Hyphomicrobium zavarzinii |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADP+ | no reduction of NAD+ | Hyphomicrobium zavarzinii | |
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Release 2023.1 (January 2023)
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