Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | not inhibited by 50 mM NH4Cl or amino-oxyacetate | Zea mays |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.8 | - |
2-oxoglutarate | - |
Zea mays | |
5.2 | - |
L-lysine | - |
Zea mays |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-lysine + 2-oxoglutarate + NADPH | Zea mays | lysine catabolism | N6-(L-1,3-dicarboxypropyl)-L-lysine + NADP+ + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Zea mays | - |
- |
- |
Purification (Comment) | Organism |
---|---|
partial purification | Zea mays |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
endosperm | during development | Zea mays | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Zea mays |
Storage Stability | Organism |
---|---|
-20°C, 2 months, no loss of activity | Zea mays |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-lysine + 2-oxoglutarate + NADPH | specific for L-lysine, 2-oxoglutarate and NADPH | Zea mays | N6-(L-1,3-dicarboxypropyl)-L-lysine + NADP+ + H2O | N6-(L-1,3-dicarboxypropyl)-L-lysine is identical with saccharopine | ? | |
L-lysine + 2-oxoglutarate + NADPH | enzyme catalyzes lysine degradation to saccharopine | Zea mays | N6-(L-1,3-dicarboxypropyl)-L-lysine + NADP+ + H2O | N6-(L-1,3-dicarboxypropyl)-L-lysine is identical with saccharopine | ? | |
L-lysine + 2-oxoglutarate + NADPH | lysine catabolism | Zea mays | N6-(L-1,3-dicarboxypropyl)-L-lysine + NADP+ + H2O | - |
? | |
additional information | not as electron acceptor: NADH | Zea mays | ? | - |
? | |
additional information | not as substrates: L-ornithine, D-lysine, oxaloacetate, pyruvate | Zea mays | ? | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
- |
Zea mays |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Zea mays |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | NADH can not replace NADPH | Zea mays | |
NADPH | specific for NADPH | Zea mays |