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Literature summary for 1.5.1.8 extracted from

  • Fjellstedt, T.A.; Robinson, J.C.
    Purification and properties of L-lysine-alpha-ketoglutarate reductase from human placenta (1975), Arch. Biochem. Biophys., 168, 536-548.
    View publication on PubMed
Search for more literature for 1.5.1.8

Activating Compound

Activating Compound Comment Organism Structure
additional information L-lysine formation is not activated by (NH4)2SO4 Homo sapiens

General Stability

General Stability Organism
EDTA and 2-mercaptoethanol stabilize during purification Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
detergent e.g. Aerosol 22, Ultrawet 60L, deoxycholic acid, cetyltrimethyl ammonium bromide, complete inactivation Homo sapiens
L-Lysine-p-nitroanilide inhibition noncompetitive with L-lysine, competitive with 2-oxoglutarate Homo sapiens
additional information not inhibited by the detergents Lubrol PX, Tween 40, saccharopine formation is not inhibited by (NH4)2SO4 Homo sapiens
S-2-aminoethyl-L-cysteine
-
 Homo sapiens
saccharopine 1 mM, 30% inhibition, product inhibition; competitive with 2-oxoglutarate, noncompetitive with L-lysine, uncompetitive with NADPH Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
480000
-
 gel filtration, no separation of L-lysine-ketoglutarate reductase EC 1.5.1.8 and saccharopine dehydrogenase EC 1.5.1.9 Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
450fold purification, no separation of lysine-ketoglutarate reductase EC 1.5.1.8 and saccharopine dehydrogenase EC 1.5.1.9 Homo sapiens

Reaction

Reaction Comment Organism Reaction ID
N6-(L-1,3-dicarboxypropyl)-L-lysine + NADP+ + H2O = L-lysine + 2-oxoglutarate + NADPH + H+ reaction mechanism Homo sapiens
a

Source Tissue

Source Tissue Comment Organism Textmining
placenta
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Homo sapiens

Storage Stability

Storage Stability Organism
4°C, pH 7.5, 5 mM 2-mercaptoethanol, 0.1 mM EDTA, storage conditions which are most effective in preserving enzyme activity Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-lysine + 2-oxoglutarate + NADPH reverse reaction not detected Homo sapiens N6-(L-1,3-dicarboxypropyl)-L-lysine + NADP+ + H2O N6-(L-1,3-dicarboxypropyl)-L-lysine is identical with saccharopine ?
L-lysine + 2-oxoglutarate + NADPH reverse reaction not detected Homo sapiens N6-(L-1,3-dicarboxypropyl)-L-lysine + NADP+ + H2O N6-(L-1,3-dicarboxypropyl)-L-lysine is identical with saccharopine ir
L-lysine + 2-oxoglutarate + NADPH very strict substrate specificity for L-lysine, 2-oxoglutarate and NADPH Homo sapiens N6-(L-1,3-dicarboxypropyl)-L-lysine + NADP+ + H2O N6-(L-1,3-dicarboxypropyl)-L-lysine is identical with saccharopine ?
L-lysine + 2-oxoglutarate + NADPH very strict substrate specificity for L-lysine, 2-oxoglutarate and NADPH Homo sapiens N6-(L-1,3-dicarboxypropyl)-L-lysine + NADP+ + H2O N6-(L-1,3-dicarboxypropyl)-L-lysine is identical with saccharopine ir
L-lysine + 2-oxoglutarate + NADPH enzyme catalyzes lysine degradation to saccharopine Homo sapiens N6-(L-1,3-dicarboxypropyl)-L-lysine + NADP+ + H2O N6-(L-1,3-dicarboxypropyl)-L-lysine is identical with saccharopine ?
L-lysine + 2-oxoglutarate + NADPH enzyme catalyzes lysine degradation to saccharopine Homo sapiens N6-(L-1,3-dicarboxypropyl)-L-lysine + NADP+ + H2O N6-(L-1,3-dicarboxypropyl)-L-lysine is identical with saccharopine ir
additional information bifunctional enzyme with lysine-oxoglutarate reductase EC 1.5.1.8 and saccharopine dehydrogenase EC 1.5.1.9 activities Homo sapiens ?
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Homo sapiens

pH Stability

pH Stability pH Stability Maximum Comment Organism
7.5
-
 4°C, in presence of 5 mM 2-mercaptoethanol and 0.1 mM EDTA, highest stability Homo sapiens

Cofactor

Cofactor Comment Organism Structure
NADPH specific for NADPH Homo sapiens
NADPH NADPH required as cofactor Homo sapiens