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Literature summary for 1.5.1.8 extracted from

  • Miron, D.; Ben-Yaacov, S.; Karchi, H.; Galili, G.
    In vitro dephosphorylation inhibits the activity of soybean lysine-ketoglutarate reductase in a lysine-regulated manner (1997), Plant J., 12, 1453-1458.
No PubMed abstract available
Search for more literature for 1.5.1.8

Activating Compound

Activating Compound Comment Organism Structure
L-lysine excess lysine is a major stimulator of enzyme activity Nicotiana tabacum
L-lysine excess cellular lysine may stimulate activity Glycine max

Inhibitors

Inhibitors Comment Organism Structure
Alkaline phosphatase dephosphorylation with alkaline phosphatase significantly inhibits enzyme activity in presence of lysine, inhibitory effect is dramatically stimulated by lysine binding to LKR, which alters the conformation exposing phosphate residues to the surface Glycine max

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-lysine + 2-oxoglutarate + NADPH Nicotiana tabacum enzyme activity is stimulated in vivo upon treatment with protein phosphatase inhibitor N6-(L-1,3-dicarboxypropyl)-L-lysine + NADP+ + H2O
-
 ?
L-lysine + 2-oxoglutarate + NADPH Nicotiana tabacum enzyme activity is modulated in vivo by a lysine-dependent intracellular signaling cascade, mediated by Ca2+ and protein phosphorylation/dephosphorylation N6-(L-1,3-dicarboxypropyl)-L-lysine + NADP+ + H2O
-
 ?
L-lysine + 2-oxoglutarate + NADPH Glycine max enzyme activity is modulated in vivo by a lysine-dependent intracellular signaling cascade, mediated by Ca2+ and protein phosphorylation/dephosphorylation N6-(L-1,3-dicarboxypropyl)-L-lysine + NADP+ + H2O
-
 ?
L-lysine + 2-oxoglutarate + NADPH Glycine max first and major regulatory step in lysine catabolism, lysine may auto-regulate its own catabolism in vivo, plant seeds: active enzyme is a phosphoprotein, its activity is modulated by opposing actions of protein kinases and phosphatases N6-(L-1,3-dicarboxypropyl)-L-lysine + NADP+ + H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Glycine max
-
 cv. Samsun
-
Nicotiana tabacum
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
phosphoprotein active bifunctional enzyme is a phosphoprotein, phosphorylation is essential for LKR activity, but not for SDH activity, incubation with casein kinase II results in a significant phosphorylation of the bifunctional enzyme Glycine max

Purification (Commentary)

Purification (Comment) Organism
1630.5fold purification of a bifunctional protein containing lysine-ketoglutarate reductase EC 1.5.1.8 and saccharopine dehydrogenase EC 1.5.1.9 activities Glycine max

Source Tissue

Source Tissue Comment Organism Textmining
seed developing Nicotiana tabacum
-
seed developing Glycine max
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.261
-
-
 Glycine max

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-lysine + 2-oxoglutarate + NADPH enzyme catalyzes lysine degradation to saccharopine Nicotiana tabacum N6-(L-1,3-dicarboxypropyl)-L-lysine + NADP+ + H2O N6-(L-1,3-dicarboxypropyl)-L-lysine is identical with saccharopine ?
L-lysine + 2-oxoglutarate + NADPH enzyme catalyzes lysine degradation to saccharopine Glycine max N6-(L-1,3-dicarboxypropyl)-L-lysine + NADP+ + H2O N6-(L-1,3-dicarboxypropyl)-L-lysine is identical with saccharopine ?
L-lysine + 2-oxoglutarate + NADPH enzyme activity is stimulated in vivo upon treatment with protein phosphatase inhibitor Nicotiana tabacum N6-(L-1,3-dicarboxypropyl)-L-lysine + NADP+ + H2O
-
 ?
L-lysine + 2-oxoglutarate + NADPH enzyme activity is modulated in vivo by a lysine-dependent intracellular signaling cascade, mediated by Ca2+ and protein phosphorylation/dephosphorylation Nicotiana tabacum N6-(L-1,3-dicarboxypropyl)-L-lysine + NADP+ + H2O
-
 ?
L-lysine + 2-oxoglutarate + NADPH enzyme activity is modulated in vivo by a lysine-dependent intracellular signaling cascade, mediated by Ca2+ and protein phosphorylation/dephosphorylation Glycine max N6-(L-1,3-dicarboxypropyl)-L-lysine + NADP+ + H2O
-
 ?
L-lysine + 2-oxoglutarate + NADPH first and major regulatory step in lysine catabolism, lysine may auto-regulate its own catabolism in vivo, plant seeds: active enzyme is a phosphoprotein, its activity is modulated by opposing actions of protein kinases and phosphatases Glycine max N6-(L-1,3-dicarboxypropyl)-L-lysine + NADP+ + H2O
-
 ?
additional information bifunctional enzyme with lysine-oxoglutarate reductase EC 1.5.1.8 and saccharopine dehydrogenase EC 1.5.1.9 activities Glycine max ?
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Glycine max

Cofactor

Cofactor Comment Organism Structure
NADPH
-
 Nicotiana tabacum
NADPH
-
 Glycine max