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Literature summary for 1.5.3.1 extracted from

  • Ogushi, S.; Nagao, K.; Emi, S.; Ando, M.; Tsuru, D.
    Sarcosine oxidase from Arthrobacter urefaciens: Purification and some properties (1988), Chem. Pharm. Bull., 36, 1445-1450.
No PubMed abstract available

Inhibitors

Inhibitors Comment Organism Structure
2-mercaptoethanol slight Paenarthrobacter ureafaciens
Cd2+
-
Paenarthrobacter ureafaciens
dithiothreitol
-
Paenarthrobacter ureafaciens
EDTA slight Paenarthrobacter ureafaciens
iodoacetamide
-
Paenarthrobacter ureafaciens
iodoacetate
-
Paenarthrobacter ureafaciens
N-ethylmaleimide
-
Paenarthrobacter ureafaciens
o-phenanthroline
-
Paenarthrobacter ureafaciens
p-chloromercuribenzoate
-
Paenarthrobacter ureafaciens
Zn2+
-
Paenarthrobacter ureafaciens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.4
-
sarcosine
-
Paenarthrobacter ureafaciens
16.5
-
N-Methyl-DL-alanine
-
Paenarthrobacter ureafaciens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
14000
-
1 * 96000 + 1 * 45000 + 1 * 23000 + 1 * 14000, SDS-PAGE Paenarthrobacter ureafaciens
23000
-
1 * 96000 + 1 * 45000 + 1 * 23000 + 1 * 14000, SDS-PAGE Paenarthrobacter ureafaciens
45000
-
1 * 96000 + 1 * 45000 + 1 * 23000 + 1 * 14000, SDS-PAGE Paenarthrobacter ureafaciens
96000
-
1 * 96000 + 1 * 45000 + 1 * 23000 + 1 * 14000, SDS-PAGE Paenarthrobacter ureafaciens
185000
-
gel filtration Paenarthrobacter ureafaciens

Organism

Organism UniProt Comment Textmining
Paenarthrobacter ureafaciens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Paenarthrobacter ureafaciens

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
7.3
-
-
Paenarthrobacter ureafaciens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N-methyl-DL-alanine + O2 + H2O
-
Paenarthrobacter ureafaciens formaldehyde + DL-alanine + H2O2
-
?
sarcosine + O2 + H2O
-
Paenarthrobacter ureafaciens glycine + formaldehyde + H2O2
-
?

Subunits

Subunits Comment Organism
tetramer 1 * 96000 + 1 * 45000 + 1 * 23000 + 1 * 14000, SDS-PAGE Paenarthrobacter ureafaciens

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
20
-
pH 6.5-9.5, 20 h Paenarthrobacter ureafaciens
40
-
pH 8.0, 24 h, 50% loss of activity Paenarthrobacter ureafaciens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.1
-
N-Methyl-DL-alanine
-
Paenarthrobacter ureafaciens
5.8
-
sarcosine
-
Paenarthrobacter ureafaciens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.3
-
-
Paenarthrobacter ureafaciens

pH Range

pH Minimum pH Maximum Comment Organism
6 10 pH 6: about 35% of maximum activity Paenarthrobacter ureafaciens
6 10 pH 10: about 55% of maximum activity Paenarthrobacter ureafaciens

pH Stability

pH Stability pH Stability Maximum Comment Organism
6.5 9.5 20°C, stable for 20 h Paenarthrobacter ureafaciens

Cofactor

Cofactor Comment Organism Structure
flavin associated with 45000 Da subunit Paenarthrobacter ureafaciens