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Literature summary for 1.5.3.1 extracted from

  • Hassan-Abdallah, A.; Bruckner, R.C.; Zhao, G.; Jorns, M.S.
    Biosynthesis of covalently bound flavin: isolation and in vitro flavinylation of the monomeric sarcosine oxidase apoprotein (2005), Biochemistry, 44, 6452-6462.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.5.3.1

Protein Variants

Protein Variants Comment Organism
additional information production of soluble apoenzyme lacking covalently bound FAD by controlled expression in Escherichia coli. Reconstitution of enzyme by incubation with FAD Bacillus sp. (in: Bacteria)

Organism

Organism UniProt Comment Textmining
Bacillus sp. (in: Bacteria)
-
-
-

Renatured (Commentary)

Renatured (Comment) Organism
production of soluble apoenzyme lacking FAD by controlled expression in Escherichia coli. Reconstitution of enzyme by incubation with FAD. Autoflavinylation occurs in a reaction that proceeds via reduced flavin intermediate and requires only apoenzyme and FAD Bacillus sp. (in: Bacteria)

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1
-
 apoenzyme lacking FAD Bacillus sp. (in: Bacteria)
3
-
 apoenzyme reconstituted with 8-nor-8-chloro-FAD Bacillus sp. (in: Bacteria)
27
-
 apoenzyme reconstituted with FAD Bacillus sp. (in: Bacteria)
34.7
-
 native enzyme Bacillus sp. (in: Bacteria)

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
sarcosine + H2O + O2
-
 Bacillus sp. (in: Bacteria) glycine + formaldehyde + H2O2
-
 ?

Cofactor

Cofactor Comment Organism Structure
FAD covalently bound to wild-type. Production of soluble apoenzyme lacking FAD by controlled expression in Escherichia coli. Reconstitution of enzyme by incubation with FAD. Autoflavinylation occurs in a reaction that proceeds via reduced flavin intermediate and requires only apoenzyme and FAD Bacillus sp. (in: Bacteria)