Cloned (Comment) | Organism |
---|---|
expression in Pichia pastoris, wild-type and mutant enzymes | Zea mays |
Protein Variants | Comment | Organism |
---|---|---|
E170Q | mutation results in moderate change of enzyme activity and apparent Km-values | Zea mays |
E62Q | mutation results in moderate change of enzyme activity and apparent Km-values | Zea mays |
K300M | mutation results in a 1400fold decrease in the rate of flavin reduction and a 160fold decrease in the equilibrium dissociation constant for the K300M-spermidine complex, consistent with a major role for this residue in the mechanism of substrate oxidation | Zea mays |
Y298F | specific activity or KM-values are not substantially altered | Zea mays |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0007 | - |
spermidine | pH 6.0, 25°C, mutant enzyme Y298F | Zea mays | |
0.0012 | - |
spermine | pH 6.0, 25°C, mutant enzyme Y298F | Zea mays | |
0.0016 | - |
spermine | pH 6.0, 25°C, wild-type enzyme | Zea mays | |
0.0017 | - |
spermidine | pH 6.0, 25°C, native enzyme | Zea mays | |
0.0017 | - |
spermine | pH 6.0, 25°C, native enzyme | Zea mays | |
0.0021 | - |
spermidine | pH 6.0, 25°C, wild-type enzyme | Zea mays | |
0.0125 | - |
spermine | pH 6.0, 25°C, mutant enzyme E170Q | Zea mays | |
0.0147 | - |
spermine | pH 6.0, 25°C, mutant enzyme E62Q | Zea mays | |
0.0167 | - |
spermidine | pH 6.0, 25°C, mutant enzyme E170Q | Zea mays | |
0.0246 | - |
spermidine | pH 6.0, 25°C, mutant enzyme E62Q | Zea mays |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Zea mays | O64411 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Zea mays |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
seedling | - |
Zea mays | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
spermidine + O2 + H2O | - |
Zea mays | ? | - |
? | |
spermine + O2 + H2O | - |
Zea mays | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
MPAO | - |
Zea mays |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.004 | - |
spermidine | pH 6.0, 25°C, mutant enzyme K300M | Zea mays | |
0.0053 | - |
spermine | pH 6.0, 25°C, mutant enzyme K300M | Zea mays | |
2.8 | - |
spermine | pH 6.0, 25°C, mutant enzyme E62Q | Zea mays | |
4.6 | - |
spermine | pH 6.0, 25°C, mutant enzyme E170Q | Zea mays | |
7.7 | - |
spermine | pH 6.0, 25°C, mutant enzyme Y298F | Zea mays | |
8.3 | - |
spermidine | pH 6.0, 25°C, mutant enzyme E62Q | Zea mays | |
16.5 | - |
spermidine | pH 6.0, 25°C, mutant enzyme Y298F | Zea mays | |
17.3 | - |
spermidine | pH 6.0, 25°C, mutant enzyme E170Q | Zea mays | |
32.9 | - |
spermine | pH 6.0, 25°C, wild-type enzyme | Zea mays | |
39.3 | - |
spermine | pH 6.0, 25°C, native enzyme | Zea mays | |
50.2 | - |
spermidine | pH 6.0, 25°C, wild-type enzyme | Zea mays | |
55.1 | - |
spermidine | pH 6.0, 25°C, native enzyme | Zea mays |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | oxidized FAD is the prominent form during steady-state turnover, consistent with the reductive half-reaction being rate-limiting | Zea mays |