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Literature summary for 1.5.3.6 extracted from

  • Brandsch, R.; Bichler, V.
    In vivo and in vitro expression of the 6-hydroxy-D-nicotine oxidase gene of Arthrobacter oxidans, cloned into Escherichia coli, as an enzymatically active, covalently flavinylated polypeptide (1985), FEBS Lett., 192, 204-208.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli HB101 Paenarthrobacter nicotinovorans

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
Paenarthrobacter nicotinovorans 5737
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
52000
-
expression in Escherichia coli maxicells Paenarthrobacter nicotinovorans

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(R)-6-hydroxynicotine + H2O + O2 Paenarthrobacter nicotinovorans
-
1-(6-hydroxypyrid-3-yl)-4-(methylamino)-butan-1-one + H2O2
-
?
additional information Paenarthrobacter nicotinovorans enzyme production is induced by growing cells in D,L-nicotine as only source of carbon and nitrogen ?
-
?
additional information Paenarthrobacter nicotinovorans it is synthesized only during the late logarithmic or early stationary phases of growth ?
-
?

Organism

Organism UniProt Comment Textmining
Paenarthrobacter nicotinovorans
-
formerly Arthrobacter oxidans
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.06
-
expressed in Escherichia coli HB101 cells Paenarthrobacter nicotinovorans
0.08
-
wild type enzyme Paenarthrobacter nicotinovorans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(R)-6-hydroxynicotine + H2O + O2
-
Paenarthrobacter nicotinovorans 1-(6-hydroxypyrid-3-yl)-4-(methylamino)-butan-1-one + H2O2
-
?
additional information enzyme production is induced by growing cells in D,L-nicotine as only source of carbon and nitrogen Paenarthrobacter nicotinovorans ?
-
?
additional information it is synthesized only during the late logarithmic or early stationary phases of growth Paenarthrobacter nicotinovorans ?
-
?

Cofactor

Cofactor Comment Organism Structure
FAD
-
Paenarthrobacter nicotinovorans