Protein Variants | Comment | Organism |
---|---|---|
K110A | inactive | Mycobacterium tuberculosis |
Y203F | the mutant shows severely reduced catalytic efficiency compared to the wild type enzyme | Mycobacterium tuberculosis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0034 | - |
2,6-dichlorophenolindophenol | wild type enzyme, at 25°C and pH 7.1 in 20 mM Tris-HCl | Mycobacterium tuberculosis | |
0.86 | - |
L-proline | mutant enzyme Y203F, at 25°C and pH 7.1 in 20 mM Tris-HCl | Mycobacterium tuberculosis | |
5.7 | - |
L-proline | wild type enzyme, at 25°C and pH 7.1 in 20 mM Tris-HCl | Mycobacterium tuberculosis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
37000 | - |
2 * 37000, SDS-PAGE | Mycobacterium tuberculosis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycobacterium tuberculosis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
nickel affinity column chromatography | Mycobacterium tuberculosis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-proline + 2,6-dichlorophenolindophenol + H2O | - |
Mycobacterium tuberculosis | DELTA1-pyrroline-5-carboxylate + reduced 2,6-dichlorophenolindophenol | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 37000, SDS-PAGE | Mycobacterium tuberculosis |
Synonyms | Comment | Organism |
---|---|---|
L-proline dehydrogenase | - |
Mycobacterium tuberculosis |
PRODH | - |
Mycobacterium tuberculosis |
prub | - |
Mycobacterium tuberculosis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
33.5 | - |
L-proline | wild type enzyme, at 25°C and pH 7.1 in 20 mM Tris-HCl | Mycobacterium tuberculosis | |
100.6 | - |
L-proline | mutant enzyme Y203F, at 25°C and pH 7.1 in 20 mM Tris-HCl | Mycobacterium tuberculosis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Mycobacterium tuberculosis |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.117 | - |
L-proline | mutant enzyme Y203F, at 25°C and pH 7.1 in 20 mM Tris-HCl | Mycobacterium tuberculosis | |
5.877 | - |
L-proline | wild type enzyme, at 25°C and pH 7.1 in 20 mM Tris-HCl | Mycobacterium tuberculosis |