KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.01 | - |
5-methyltetrahydrofolate | - |
Clostridium formicaceticum | |
11.1 | - |
benzyl viologen | - |
Clostridium formicaceticum |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Iron | iron-sulfur protein, contains 15.2 iron and 19.5 acid labile sulfide per 237000 Da enzyme | Clostridium formicaceticum | |
Zinc | enzyme contains 2.3 zinc per 237000 Da enzyme | Clostridium formicaceticum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
26000 | - |
alpha4beta4, 4 * 26000 + 4 * 35000, SDS-PAGE | Clostridium formicaceticum |
35000 | - |
alpha4beta4, 4 * 26000 + 4 * 35000, SDS-PAGE | Clostridium formicaceticum |
237000 | - |
gel filtration | Clostridium formicaceticum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Clostridium formicaceticum | - |
- |
- |
Oxidation Stability | Organism |
---|---|
the enzyme loses 50% of the activity within 2 h in aerobic buffer (0.1 M Tris/HCl buffer, pH 7.4) with or without a reducing agent and in anaerobic buffer without a reducing agent | Clostridium formicaceticum |
Purification (Comment) | Organism |
---|---|
- |
Clostridium formicaceticum |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
139 | - |
- |
Clostridium formicaceticum |
Storage Stability | Organism |
---|---|
10°C, pH 7.4, 50 mM Tris/HCl containing 20% glycerol and 2 mM dithionite in an anaerobic chamber | Clostridium formicaceticum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5,10-methylenetetrahydrofolate + FADH2 | - |
Clostridium formicaceticum | 5,10-methylenetetrahydrofolate + FAD | - |
? | |
5,10-methylenetetrahydrofolate + reduced ferredoxin | - |
Clostridium formicaceticum | 5-methyltetrahydrofolate + ferredoxin | - |
? | |
5-methyltetrahydrofolate + benzyl viologen | - |
Clostridium formicaceticum | 5,10-methylenetetrahydrofolate + reduced benzyl viologen | - |
? | |
5-methyltetrahydrofolate + benzyl viologen | 11% of the activity with methylene blue | Clostridium formicaceticum | 5,10-methylenetetrahydrofolate + reduced benzyl viologen | - |
? | |
5-methyltetrahydrofolate + FAD | 11% of the activity with methylene blue | Clostridium formicaceticum | 5,10-methylenetetrahydrofolate + FADH2 | - |
? | |
5-methyltetrahydrofolate + menadione | 25% of the activity with methylene blue | Clostridium formicaceticum | 5,10-methylenetetrahydrofolate + reduced menadiol | - |
? | |
5-methyltetrahydrofolate + rubredoxin | 11% of the activity with methylene blue | Clostridium formicaceticum | 5,10-methylenetetrahydrofolate + reduced rubredoxin | - |
? | |
additional information | no activity with pyridine nucleotides | Clostridium formicaceticum | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
octamer | alpha4beta4, 4 * 26000 + 4 * 35000, SDS-PAGE | Clostridium formicaceticum |
Synonyms | Comment | Organism |
---|---|---|
5,10-methylenetetrahydrofolate reductase | - |
Clostridium formicaceticum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.8 | 8 | - |
Clostridium formicaceticum |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
7.1 | 7.6 | the enzyme is more stable in Tris/HCl than in triethanolamine/HCl or phosphate buffer | Clostridium formicaceticum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | contains 1.7 FAD per 237000 Da enzyme | Clostridium formicaceticum |