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Literature summary for 1.5.98.1 extracted from

  • Schwörer, B.; Breitung, J.; Klein, A.R.; Stetter, K.O.; Thauer, R.K.
    Formylmethanofuran:tetrahydromethanopterin formyltransferase and N5,N10-methylenetetrahydromethanopterin dehydrogenase from the sulfate-reducing Archaeoglobus fulgidus: similarities with the enzymes from methanogenic Archaea (1993), Arch. Microbiol., 159, 225-232.
    View publication on PubMed
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General Stability

General Stability Organism
thermostable up to 90°C, only in the presence of salts. Complete stability in presence of 0.4-0.5 M K2HPO4 Archaeoglobus fulgidus

Inhibitors

Inhibitors Comment Organism Structure
(NH4)2SO4 0.15M-0.2M (NH4)2SO4 stimulates by a factor of 1.3. Higher concentrations are inhibitory Archaeoglobus fulgidus
K2HPO4 0.15M-0.2M K2HPO4 stimulates by a factor of 1.3. Higher concentrations are inhibitory Archaeoglobus fulgidus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.013
-
 coenzyme F420
-
 Archaeoglobus fulgidus
0.017
-
 5,10-methylenetetrahydromethanopterin
-
 Archaeoglobus fulgidus

Metals/Ions

Metals/Ions Comment Organism Structure
(NH4)2SO4 0.15M-0.2M (NH4)2SO4 stimulates by a factor of 1.3. Higher concentrations are inhibitory Archaeoglobus fulgidus
K+ 0.15M-0.2M K2HPO4 stimulates by a factor of 1.3. Higher concentrations are inhibitory Archaeoglobus fulgidus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
32000
-
 x * 32000, SDS-PAGE Archaeoglobus fulgidus
140000
-
 gel filtration Archaeoglobus fulgidus

Organism

Organism UniProt Comment Textmining
Archaeoglobus fulgidus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Archaeoglobus fulgidus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1560
-
-
 Archaeoglobus fulgidus

Storage Stability

Storage Stability Organism
-20°C, stable for several weeks Archaeoglobus fulgidus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5,10-methylenetetrahydromethanopterin + oxidized coenzyme F420 r Archaeoglobus fulgidus 5,10-methenyltetrahydromethanopterin + reduced coenzyme F420
-
 ?
5,10-methylenetetrahydromethanopterin + oxidized coenzyme F420 r Archaeoglobus fulgidus VC-19 / DSM 4304 5,10-methenyltetrahydromethanopterin + reduced coenzyme F420
-
 ?

Subunits

Subunits Comment Organism
? x * 32000, SDS-PAGE Archaeoglobus fulgidus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
70
-
-
 Archaeoglobus fulgidus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
90
-
 thermostable up to 90°C, only in the presence of salts. Complete stability in presence of 0.4-0.5 M K2HPO4 Archaeoglobus fulgidus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5
-
-
 Archaeoglobus fulgidus

Cofactor

Cofactor Comment Organism Structure
coenzyme F420 required Archaeoglobus fulgidus