Crystallization (Comment) | Organism |
---|---|
purified recombinant selenomethionine-labeled enzyme, hanging drop vapour diffusion method, 0.001 ml protein solution containing 12 mg/ml enzyme and 10 mM MOPS-KOH, pH 7.0, mixed with 0.001 ml reservoir solution containing 13% v/v 2-methyl-2,4-pentanediol, 0.1 M sodium phosphate, pH 8.0, and 0.2 M magnesium acetate, X-ray diffraction structure determination and analysis at 1.54 A resolution by single wavelength anomalous dispersion method, or at 2.4 A resolution by multiwavelength anomalous dispersion method | Methanopyrus kandleri |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | ternary complex kinetic mechanism | Methanopyrus kandleri |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
5,10-methylenetetrahydromethanopterin + oxidized coenzyme F420 | Methanopyrus kandleri | fourth step in the CO2 reduction to methane | 5,10-methenyltetrahydromethanopterin + reduced coenzyme F420 | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methanopyrus kandleri | - |
methanogenic archeon | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5,10-methylenetetrahydromethanopterin + oxidized coenzyme F420 | fourth step in the CO2 reduction to methane | Methanopyrus kandleri | 5,10-methenyltetrahydromethanopterin + reduced coenzyme F420 | - |
r | |
5,10-methylenetetrahydromethanopterin + oxidized coenzyme F420 | substrate binding site structure analysis | Methanopyrus kandleri | 5,10-methenyltetrahydromethanopterin + reduced coenzyme F420 | - |
r |
Subunits | Comment | Organism |
---|---|---|
hexamer | 6 * 30000-35000, homohexamer build as a trimer of dimers, each subunit has 2 domains, a larger alphabeta domain and a smaller helix bundle domain, quarternary structure from crystal strutcure analysis | Methanopyrus kandleri |
More | structural adaption to extreme environment | Methanopyrus kandleri |
Synonyms | Comment | Organism |
---|---|---|
MTD | - |
Methanopyrus kandleri |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
coenzyme F420 | dependent on, binding site structure | Methanopyrus kandleri | |
additional information | enzyme contains no prosthetic group | Methanopyrus kandleri |