Cloned (Comment) | Organism |
---|---|
overexpression in yeast Yarrowia lipolytica | Zea mays |
Crystallization (Comment) | Organism |
---|---|
purified recombinant enzyme, 20 mg/ml protein with 0.5% w/v n-octyl-beta-D-glucopyranoside, hanging drop vapour diffusion method, 0.002 ml protein solution mixed with 0.002 ml reservoir solution containing 30% w/v PEG 1500, 0.1 M Tris-HCl, pH 7.0, 1 week, 20°C, X-ray diffraction structure determination and analysis at 1.95 A resolution | Zea mays |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
63000 | - |
x * 69000, recombinant enzyme, SDS-PAGE, x * 63000, native enzyme, SDS-PAGE | Zea mays |
69000 | - |
x * 69000, recombinant enzyme, SDS-PAGE, x * 63000, native enzyme, SDS-PAGE | Zea mays |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
N6-(DELTA2-isopentenyl)adenine + FAD + H2O | Zea mays | oxidative degradation of cytokinins | adenine + 3-methylbut-2-enal + FADH2 | - |
ir |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Zea mays | - |
enzyme ZmCKO1 | - |
Purification (Comment) | Organism |
---|---|
recombinant from yeast Yarrowia lipolytica by ammonium sulfate fractionation, gel filtration, and ion exchange chromatography | Zea mays |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
30.6 | - |
purified recombinant enzyme | Zea mays |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
N6-(DELTA2-isopentenyl)adenine + FAD + H2O | - |
Zea mays | adenine + 3-methylbut-2-enal + FADH2 | - |
ir | |
N6-(DELTA2-isopentenyl)adenine + FAD + H2O | oxidative degradation of cytokinins | Zea mays | adenine + 3-methylbut-2-enal + FADH2 | - |
ir |
Subunits | Comment | Organism |
---|---|---|
? | x * 69000, recombinant enzyme, SDS-PAGE, x * 63000, native enzyme, SDS-PAGE | Zea mays |
Synonyms | Comment | Organism |
---|---|---|
CKO | - |
Zea mays |
cytokinin oxidase/dehydrogenase | - |
Zea mays |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | covalently bound | Zea mays |