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Literature summary for 1.5.99.12 extracted from
- Rate enhancement of cytokinin oxidase/dehydrogenase using 2,6-dichlorophenolindophenol as an electron acceptor (2003), Plant Growth Regul., 40, 189-196.
No PubMed abstract available
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| functional expression in Pichia pastoris | Zea mays |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| N6-dimethylallyladenine + FAD + H2O | Zea mays | - |
adenine + 3-methylbut-2-enal + FADH2 | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Zea mays | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partially, recombinant enzyme from Pichia pastoris | Zea mays |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
assay development and optimization with 2,6-dichlorophenolindophenol as electron acceptor | Zea mays |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| N6-dimethylallyladenine + 2,6-dichlorophenolindophenol | i.e. isopentenyladenine, reaction via an imine intermediate | Zea mays | adenine + 3-methylbut-2-enal + reduced 2,6-dichlorophenolindophenol | - |
? | |
| N6-dimethylallyladenine + FAD + H2O | - |
Zea mays | adenine + 3-methylbut-2-enal + FADH2 | - |
? | |
| N6-dimethylallyladenine + FAD + H2O2 | i.e. isopentenyladenine | Zea mays | adenine + 3-methylbut-2-enal + FADH2 + O2 | - |
? | |
| zeatin + 2,6-dichlorophenolindophenol | - |
Zea mays | ? + reduced 2,6-dichlorophenolindophenol | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| cytokinin oxidase/dehydrogenase | - |
Zea mays |
| ZmCKX1 | - |
Zea mays |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 22 | - |
assay at room temperature | Zea mays |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
assay at | Zea mays |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| 2,6-dichlorophenolindophenol | in vitro 4000fold faster reaction than with the native acceptor | Zea mays | |
| FAD | - |
Zea mays | |
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Release 2023.1 (January 2023)
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