Crystallization (Comment) | Organism |
---|---|
hanging-drop vapor-diffusion method in the presence of N6-(buta-2,3-dienyl)adenine or N6-(penta-2,3-dienyl)adenine | Zea mays |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
N6-(2-bromo-2-isopentenyl)adenine | weak inhibition | Zea mays | |
N6-(2-butynyl)adenine | irreversible inhibition | Zea mays | |
N6-(4-hydroxy-2-butynyl)adenine | irreversible inhibition | Zea mays | |
N6-(buta-2,3-dienyl)adenine | strong irreversible mechanism based inhibition | Zea mays | |
N6-(penta-2,3-dienyl)adenine | strong irreversible mechanism based inhibition | Zea mays |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0011 | - |
N6-(2-isopentenyl)adenine | 37°C | Zea mays | |
0.092 | - |
N6-(2-butynyl)adenine | 37°C | Zea mays | |
0.38 | - |
N6-(4-hydroxy-2-butynyl)adenine | 37°C | Zea mays |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
69865 | - |
1 * 69865, MALDI-TOF MS, crystal structure analysis | Zea mays |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Zea mays | irreversible oxidative cleavage of the N6-side chain of cytokinins, products are adenine and side-chain derived aldehyde, important for the regulation of early development of the plant embryo | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Zea mays | Q9T0N8 | - |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | - |
Zea mays |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
embryo | strong expression in kernel | Zea mays | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | irreversible oxidative cleavage of the N6-side chain of cytokinins, products are adenine and side-chain derived aldehyde, important for the regulation of early development of the plant embryo | Zea mays | ? | - |
? | |
N6-(2-butynyl)adenine + 2,6-dichlorophenol indophenol | very effective electron acceptor | Zea mays | ? | - |
? | |
N6-(2-isopentenyl)adenine + 2,6-dichlorophenol indophenol | very effective electron acceptor | Zea mays | ? | - |
? | |
N6-(2-isopentenyl)adenosine + 2,6-dichlorophenol indophenol | very effective electron acceptor | Zea mays | ? | - |
? | |
N6-(4-hydroxy-2-butynyl)adenine + 2,6-dichlorophenol indophenol | very effective electron acceptor | Zea mays | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 69865, MALDI-TOF MS, crystal structure analysis | Zea mays |
Synonyms | Comment | Organism |
---|---|---|
CKX | - |
Zea mays |
cytokinin oxidase/dehydrogenase | - |
Zea mays |
ZmCKO1 | - |
Zea mays |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Zea mays |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.01 | - |
N6-(buta-2,3-dienyl)adenine | - |
Zea mays | |
0.026 | - |
N6-(penta-2,3-dienyl)adenine | - |
Zea mays |