Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli | Thermus thermophilus |
Crystallization (Comment) | Organism |
---|---|
sitting drop vapour diffusion method, with 100 mM imidazole (pH 7), 100 mM MgCl2, 17% 2-methyl-2,4-pentanediol, and 5 mM dithiothreitol | Thermus thermophilus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
L-lactate | - |
Thermus thermophilus | |
L-Mandelate | - |
Thermus thermophilus | |
L-tetrahydro-2-furoic acid | competitive inhibitor | Thermus thermophilus | |
additional information | trans-4-hydroxy-L-proline, cis-4-hydroxy-L-proline, L-azetidine-2-carboxylic acid, and L-pipecolinic acid are no inhibitors of PRODH | Thermus thermophilus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
4 | - |
3,4-dehydro-L-proline | in 50 mM Tris-HCl, at 25°C | Thermus thermophilus | |
13 | - |
L-proline | with O2 as cosubstrate, in 50 mM Tris-HCl, at 25°C | Thermus thermophilus | |
27 | - |
L-proline | with 2,6-dichlorophenolindophenol as cosubstrate, in 50 mM Tris-HCl, at 25°C | Thermus thermophilus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | - |
Thermus thermophilus | 5739 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
37970 | - |
MALDI-TOF mass spectrometry | Thermus thermophilus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermus thermophilus | Q72IB8 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography and Sephadex G-25 gel filtration | Thermus thermophilus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3,4-dehydro-L-proline + acceptor + H2O | efficient substrate | Thermus thermophilus | ? + reduced acceptor | - |
? | |
L-proline + 2,6-dichlorophenolindophenol + H2O | - |
Thermus thermophilus | (S)-1-pyrroline-5-carboxylate + reduced 2,6-dichlorophenolindophenol | - |
? | |
L-proline + cytochrome c + H2O | - |
Thermus thermophilus | (S)-1-pyrroline-5-carboxylate + reduced cytochrome c | - |
? | |
L-proline + H2O | - |
Thermus thermophilus | (S)-1-pyrroline-5-carboxylate + H2O2 | - |
? | |
additional information | trans-4-hydroxy-L-proline, cis-4-hydroxy-L-proline, L-azetidine-2-carboxylic acid, and L-pipecolinic acid are no substrates of PRODH | Thermus thermophilus | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
PRODH | - |
Thermus thermophilus |
proline dehydrogenase | - |
Thermus thermophilus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
90 | - |
PRODH exhibits over 85% residual activity after a 1 h incubation at 90°C and over 60% residual activity after 3 h | Thermus thermophilus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.212 | - |
L-proline | with O2 as cosubstrate, in 50 mM Tris-HCl, at 25°C | Thermus thermophilus | |
13 | - |
L-proline | with 2,6-dichlorophenolindophenol as cosubstrate, in 50 mM Tris-HCl, at 25°C | Thermus thermophilus | |
75 | - |
3,4-dehydro-L-proline | in 50 mM Tris-HCl, at 25°C | Thermus thermophilus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Thermus thermophilus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
1 | - |
L-tetrahydro-2-furoic acid | in 50 mM Tris-HCl, at 25°C | Thermus thermophilus | |
2.4 | - |
L-Mandelate | in 50 mM Tris-HCl, at 25°C | Thermus thermophilus | |
2.5 | - |
L-lactate | in 50 mM Tris-HCl, at 25°C | Thermus thermophilus |