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Literature summary for 1.5.99.B2 extracted from

  • White, T.A.; Krishnan, N.; Becker, D.F.; Tanner, J.J.
    Structure and kinetics of monofunctional proline dehydrogenase from Thermus thermophilus (2007), J. Biol. Chem., 282, 14316-14327.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Thermus thermophilus

Crystallization (Commentary)

Crystallization (Comment) Organism
sitting drop vapour diffusion method, with 100 mM imidazole (pH 7), 100 mM MgCl2, 17% 2-methyl-2,4-pentanediol, and 5 mM dithiothreitol Thermus thermophilus

Inhibitors

Inhibitors Comment Organism Structure
L-lactate
-
Thermus thermophilus
L-Mandelate
-
Thermus thermophilus
L-tetrahydro-2-furoic acid competitive inhibitor Thermus thermophilus
additional information trans-4-hydroxy-L-proline, cis-4-hydroxy-L-proline, L-azetidine-2-carboxylic acid, and L-pipecolinic acid are no inhibitors of PRODH Thermus thermophilus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4
-
3,4-dehydro-L-proline in 50 mM Tris-HCl, at 25°C Thermus thermophilus
13
-
L-proline with O2 as cosubstrate, in 50 mM Tris-HCl, at 25°C Thermus thermophilus
27
-
L-proline with 2,6-dichlorophenolindophenol as cosubstrate, in 50 mM Tris-HCl, at 25°C Thermus thermophilus

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion
-
Thermus thermophilus 5739
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
37970
-
MALDI-TOF mass spectrometry Thermus thermophilus

Organism

Organism UniProt Comment Textmining
Thermus thermophilus Q72IB8
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography and Sephadex G-25 gel filtration Thermus thermophilus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3,4-dehydro-L-proline + acceptor + H2O efficient substrate Thermus thermophilus ? + reduced acceptor
-
?
L-proline + 2,6-dichlorophenolindophenol + H2O
-
Thermus thermophilus (S)-1-pyrroline-5-carboxylate + reduced 2,6-dichlorophenolindophenol
-
?
L-proline + cytochrome c + H2O
-
Thermus thermophilus (S)-1-pyrroline-5-carboxylate + reduced cytochrome c
-
?
L-proline + H2O
-
Thermus thermophilus (S)-1-pyrroline-5-carboxylate + H2O2
-
?
additional information trans-4-hydroxy-L-proline, cis-4-hydroxy-L-proline, L-azetidine-2-carboxylic acid, and L-pipecolinic acid are no substrates of PRODH Thermus thermophilus ?
-
?

Synonyms

Synonyms Comment Organism
PRODH
-
Thermus thermophilus
proline dehydrogenase
-
Thermus thermophilus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
90
-
PRODH exhibits over 85% residual activity after a 1 h incubation at 90°C and over 60% residual activity after 3 h Thermus thermophilus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.212
-
L-proline with O2 as cosubstrate, in 50 mM Tris-HCl, at 25°C Thermus thermophilus
13
-
L-proline with 2,6-dichlorophenolindophenol as cosubstrate, in 50 mM Tris-HCl, at 25°C Thermus thermophilus
75
-
3,4-dehydro-L-proline in 50 mM Tris-HCl, at 25°C Thermus thermophilus

Cofactor

Cofactor Comment Organism Structure
FAD
-
Thermus thermophilus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1
-
L-tetrahydro-2-furoic acid in 50 mM Tris-HCl, at 25°C Thermus thermophilus
2.4
-
L-Mandelate in 50 mM Tris-HCl, at 25°C Thermus thermophilus
2.5
-
L-lactate in 50 mM Tris-HCl, at 25°C Thermus thermophilus