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Literature summary for 1.5.99.B2 extracted from
- Process integration for the recovery and purification of recombinant Pseudomonas fluorescens proline dehydrogenase using aqueous two-phase systems (2013), J. Chromatogr. B, 929, 11-17.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21 (DE3) pLysS cells | Pseudomonas fluorescens |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 40000 | - |
x * 40000, SDS-PAGE | Pseudomonas fluorescens |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas fluorescens | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Pseudomonas fluorescens |
Renatured (Commentary)
| Renatured (Comment) | Organism |
|---|---|
- |
Pseudomonas fluorescens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-proline + 2-(4-iodophenyl)-3-(4-nitrophenyl)-5-phenyltetrazolium + H2O | - |
Pseudomonas fluorescens | DELTA1-pyrroline-5-carboxylate + reduced 2-(4-iodophenyl)-3-(4-nitrophenyl)-5-phenyltetrazolium | - |
? |  |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 40000, SDS-PAGE | Pseudomonas fluorescens |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| PRODH | - |
Pseudomonas fluorescens |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FAD | - |
Pseudomonas fluorescens | |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Pseudomonas fluorescens | isoelectric focusing | - |
7 |
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