Inhibitors | Comment | Organism | Structure |
---|---|---|---|
p-Aminophenylarsenoxide | 0.1 mM, 40-60% inhibition in the absence of either phosphate or magnesium ions, reduction of NAD+ by NADPH in cell-free extracts is rapidly and completely inhibited in the presence of 20 mM phosphate | Azotobacter vinelandii | |
p-chloromercuribenzoate | 0.044 mM, 40-50% inhibition after 30 min, activity can be restored by adding 2-mercaptoethanol | Azotobacter vinelandii | |
p-hydroxymercuribenzoate | - |
Azotobacter vinelandii | |
phosphate | 10-25 mM, 60-70% inhibition of purified enzyme, complete inhibition of enzyme in cell-free extracts by 5-10 mM phosphate, NADP+ reduction by NADH is inhibited, reduction of NAD+ by NADPH is hardly affected | Azotobacter vinelandii |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 10-20fold activation of NAD+ reduction by NADPH at alkaline pH in cell-free extracts | Azotobacter vinelandii | |
Mg2+ | 10-20fold activation of NAD+ reduction by NADPH at alkaline pH in cell-free extracts | Azotobacter vinelandii |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
421000 | - |
purified enzyme: exceptional stability of polymers at neutral pH | Azotobacter vinelandii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Azotobacter vinelandii | - |
- |
- |
Subunits | Comment | Organism |
---|---|---|
octamer | octameric in cell-free extract, polymeric in purified form | Azotobacter vinelandii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | 8 | - |
Azotobacter vinelandii |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
8.4 | 8.7 | half maximal activity in this pH range in the absence of Ca2+ or Mg2+, no activity above | Azotobacter vinelandii |
9.1 | 9.3 | half maximal activity in this pH range in the presence of 5 mM Ca2+ or Mg2+, no activity above | Azotobacter vinelandii |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
7 | - |
polymeric enzyme stable | Azotobacter vinelandii |