Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Pseudomonas fluorescens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
54000 | - |
x * 54000, SDS-PAGE | Pseudomonas fluorescens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas fluorescens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
affinity chromatography, native and recombinant enzyme | Pseudomonas fluorescens |
Storage Stability | Organism |
---|---|
-20°C, 50 mM Tris-HCl, pH 7.0, 2 mM dithiothreitol, several weeks, no loss of activity, great loss of activity after several months | Pseudomonas fluorescens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
NADP+ + NADH | - |
Pseudomonas fluorescens | NADPH + NAD+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
octamer | x * 54000, SDS-PAGE | Pseudomonas fluorescens |