Cloned (Comment) | Organism |
---|---|
expression of wild-type, K110A, K110M and K110R mutant enzymes in Escherichia coli | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
K110A | 200fold elevated Km value for NADH, 85% of kcat | Homo sapiens |
K110M | 1120fold elevated Km value for NADH | Homo sapiens |
K110R | similar kinetic properties as wild-type | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0006 | - |
NADH | recombinant wild-type enzyme | Homo sapiens | |
0.0023 | - |
NADH | recombinant K110R mutant enzyme | Homo sapiens | |
0.0066 | - |
ferricytochrome b5 | recombinant wild-type enzyme | Homo sapiens | |
0.0142 | - |
ferricytochrome b5 | recombinant K110A mutant enzyme | Homo sapiens | |
0.0167 | - |
ferricytochrome b5 | recombinant K110R mutant enzyme | Homo sapiens | |
0.0285 | - |
ferricytochrome b5 | recombinant K110M mutant enzyme | Homo sapiens | |
0.121 | - |
NADH | recombinant K110A mutant enzyme | Homo sapiens | |
0.672 | - |
NADH | recombinant K110M mutant enzyme | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 ferricytochrome b5 + NADH | Homo sapiens | enzyme participates in methemoglobin reduction in erythrocytes, in other tissues it plays a role in elongation and desaturation of fatty acids, P-450 mediated drug metabolism and cholesterol biosynthesis as part of the microsomal electron transfer system | 2 ferrocytochrome b5 + NAD+ + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant wild-type, K110A, K110M and K110R mutant enzyme | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 ferricytochrome b5 + NADH | enzyme participates in methemoglobin reduction in erythrocytes, in other tissues it plays a role in elongation and desaturation of fatty acids, P-450 mediated drug metabolism and cholesterol biosynthesis as part of the microsomal electron transfer system | Homo sapiens | 2 ferrocytochrome b5 + NAD+ + H+ | - |
? | |
NADH + ferricytochrome b5 | - |
Homo sapiens | NAD+ + H+ + ferrocytochrome b5 | - |
r |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
563 | - |
ferricytochrome b5 | recombinant K110M mutant enzyme | Homo sapiens | |
742 | - |
ferricytochrome b5 | recombinant K110A mutant enzyme | Homo sapiens | |
872 | - |
ferricytochrome b5 | recombinant wild-type enzyme | Homo sapiens | |
877 | - |
ferricytochrome b5 | recombinant K110R mutant enzyme | Homo sapiens |