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Literature summary for 1.6.2.2 extracted from
- Purification and characterization of cytochrome b5 reductase from the house fly, Musca domestica (1996), Comp. Biochem. Physiol. B, 113, 175-183.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.013 | - |
ferricytochrome b5 | - |
Musca domestica |  |
| 0.017 | - |
ferricyanide | - |
Musca domestica | |
| 0.044 | - |
NADH | - |
Musca domestica | |
| 0.83 | - |
oxidized 2,6-dichlorophenolindophenol | - |
Musca domestica | |
| 14 | - |
ferricytochrome b5 | - |
Musca domestica | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| endoplasmic reticulum | - |
Musca domestica | 5783 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 31000 | - |
x * 31000, may occur from protease cleavage of the 33000 Da protein during purification | Musca domestica |
| 33000 | - |
x * 33000, SDS-PAGE | Musca domestica |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Musca domestica | - |
learn-pyrethroid-resistance strain, house fly | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Triton X-100, DEAE-cellulose, CM-cellulose, 5'-ADP-agarose | Musca domestica |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 2.042 | - |
reduction of cytochrome b5 | Musca domestica |
| 1007 | - |
reduction of ferricyanide | Musca domestica |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2 ferricytochrome b5 + NADH | uses both NADH and NADPH as electron donors, artificial acceptors: ferricyanide, 2,6-dichlophenolindophenol | Musca domestica | 2 ferrocytochrome b5 + NAD+ + H+ | - |
? | |
| NADH + ferricyanide | - |
Musca domestica | NAD+ + H+ + ferrocyanide | - |
r | |
| NADH + ferricytochrome b5 | - |
Musca domestica | NAD+ + H+ + ferrocytochrome b5 | - |
r | |
| NADH + oxidized 2,6-dichlorophenolindophenol | - |
Musca domestica | NAD+ + H+ + reduced 2,6-dichlorophenolindophenol | - |
r | |
| NADPH + ferricytochrome b5 | - |
Musca domestica | NADP+ + H+ + ferrocytochrome b5 | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 33000, SDS-PAGE | Musca domestica |
| ? | x * 31000, may occur from protease cleavage of the 33000 Da protein during purification | Musca domestica |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 50.2 | - |
2,6-dichlorophenolindophenol | - |
Musca domestica | |
| 297 | - |
NADPH | - |
Musca domestica | |
| 307 | - |
ferricyanide | - |
Musca domestica | |
| 332 | - |
NADH | - |
Musca domestica | |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADPH | - |
Musca domestica | |
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