Cloned (Comment) | Organism |
---|---|
expression of wild-type, K97A, K97R, S99A, S99T, S99V, R63A, R63Q, R63K, Y65A and Y65F mutant enzymes in Escherichia coli | Sus scrofa |
Protein Variants | Comment | Organism |
---|---|---|
K97A | mutation in flavin-binding motif | Sus scrofa |
K97R | mutation in flavin-binding motif | Sus scrofa |
R63A | mutation in flavin-binding motif | Sus scrofa |
R63K | mutation in flavin-binding motif | Sus scrofa |
R63Q | mutation in flavin-binding motif | Sus scrofa |
S99A | mutation in flavin-binding motif | Sus scrofa |
S99T | mutation in flavin-binding motif | Sus scrofa |
S99V | mutation in flavin-binding motif | Sus scrofa |
Y65A | mutation in flavin-binding motif | Sus scrofa |
Y65F | mutation in flavin-binding motif | Sus scrofa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00148 | - |
ferricytochrome b5 | S99A mutant enzyme | Sus scrofa | |
0.0019 | - |
NADH | S99T mutant enzyme | Sus scrofa | |
0.0021 | - |
NADH | R63K mutant enzyme | Sus scrofa | |
0.0029 | - |
NADH | K97R mutant enzyme | Sus scrofa | |
0.003 | - |
NADH | Y65F mutant enzyme | Sus scrofa | |
0.0031 | - |
NADH | recombinant wild-type enzyme | Sus scrofa | |
0.0042 | - |
NADH | Y65A mutant enzyme | Sus scrofa | |
0.0044 | - |
NADH | K97A mutant enzyme | Sus scrofa | |
0.005 | - |
ferricytochrome b5 | Y65A mutant enzyme | Sus scrofa | |
0.0062 | - |
ferricytochrome b5 | Y65F mutant enzyme | Sus scrofa | |
0.0069 | - |
ferricytochrome b5 | K97R mutant enzyme | Sus scrofa | |
0.0073 | - |
ferricytochrome b5 | R63K mutant enzyme | Sus scrofa | |
0.0094 | - |
NADH | S99V mutant enzyme | Sus scrofa | |
0.0099 | - |
ferricytochrome b5 | S99V mutant enzyme | Sus scrofa | |
0.0104 | - |
ferricytochrome b5 | recombinant wild-type enzyme | Sus scrofa | |
0.0118 | - |
ferricytochrome b5 | S99T mutant enzyme | Sus scrofa | |
0.0161 | - |
ferricytochrome b5 | K97A mutant enzyme | Sus scrofa | |
0.0185 | - |
ferricytochrome b5 | R63Q mutant enzyme | Sus scrofa | |
0.021 | - |
NADH | R63Q mutant enzyme | Sus scrofa | |
0.026 | - |
NADH | S99A mutant enzyme | Sus scrofa | |
0.0421 | - |
ferricytochrome b5 | R63A mutant enzyme | Sus scrofa | |
0.104 | - |
NADH | R63A mutant enzyme | Sus scrofa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sus scrofa | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant wild-type, K97A, K97R, S99A, S99T, S99V, R63A, R63Q, R63K, Y65A and Y65F mutant enzymes | Sus scrofa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
NADH + ferricytochrome b5 | - |
Sus scrofa | NAD+ + H+ + ferrocytochrome b5 | - |
r |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
105 | - |
ferricytochrome b5 | R63Q mutant enzyme | Sus scrofa | |
107 | - |
ferricytochrome b5 | Y65A mutant enzyme | Sus scrofa | |
121 | - |
ferricytochrome b5 | R63A mutant enzyme | Sus scrofa | |
165 | - |
ferricytochrome b5 | S99V mutant enzyme | Sus scrofa | |
180 | - |
NADH | Y65A mutant enzyme | Sus scrofa | |
191 | - |
ferricytochrome b5 | K97A mutant enzyme | Sus scrofa | |
295 | - |
ferricytochrome b5 | S99A mutant enzyme | Sus scrofa | |
305 | - |
ferricytochrome b5 | Y65F mutant enzyme | Sus scrofa | |
347 | - |
ferricytochrome b5 | R63K mutant enzyme | Sus scrofa | |
375 | - |
NADH | S99V mutant enzyme | Sus scrofa | |
403 | - |
NADH | R63A mutant enzyme | Sus scrofa | |
412 | - |
NADH | R63Q mutant enzyme | Sus scrofa | |
415 | - |
ferricytochrome b5 | K97R mutant enzyme | Sus scrofa | |
435 | - |
NADH | S99A mutant enzyme | Sus scrofa | |
501 | - |
NADH | Y65F mutant enzyme | Sus scrofa | |
515 | - |
NADH | R63K mutant enzyme | Sus scrofa | |
520 | - |
ferricytochrome b5 | S99T mutant enzyme | Sus scrofa | |
661 | - |
ferricytochrome b5 | recombinant wild-type enzyme | Sus scrofa | |
851 | - |
NADH | S99T mutant enzyme | Sus scrofa | |
909 | - |
NADH | K97R mutant enzyme | Sus scrofa | |
1060 | - |
NADH | K97A mutant enzyme | Sus scrofa | |
1100 | - |
NADH | recombinant wild-type enzyme | Sus scrofa |