Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3)-RIL | Rattus norvegicus |
Protein Variants | Comment | Organism |
---|---|---|
L148P | 31% of wild type activity, reduced temperature stability and resistance against limited proteolysis with trypsin, increased affinity for NAD+ | Rattus norvegicus |
P144L | 28% of wild type activity, reduced temperature stability and resistance against limited proteolysis with trypsin, increased affinity for NAD+ | Rattus norvegicus |
P144L/L148P | 8% of wild type activity, reduced temperature stability and resistance against limited proteolysis with trypsin, increased affinity for NAD+ | Rattus norvegicus |
R159 | deletion mutant, could not be successfully expressed | Rattus norvegicus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
NAD+ | competitive, stronger inhibition of mutant enzymes compared to wild type enzyme | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.008 | - |
ferricyanide | L148P mutant enzyme, pH 7.0, 25°C | Rattus norvegicus | |
0.008 | - |
ferricyanide | P144L mutant enzyme, pH 7.0, 25°C | Rattus norvegicus | |
0.008 | - |
ferricyanide | P144L/L148P mutant enzyme, pH 7.0, 25°C | Rattus norvegicus | |
0.008 | - |
ferricyanide | wild type enzyme, pH 7.0, 25°C | Rattus norvegicus | |
0.01 | - |
ferricytochome b5 | L148P mutant enzyme, pH 7.0, 25°C | Rattus norvegicus | |
0.012 | - |
ferricytochome b5 | P144L mutant enzyme, pH 7.0, 25°C | Rattus norvegicus | |
0.013 | - |
ferricytochome b5 | P144L/L148P mutant enzyme, pH 7.0, 25°C | Rattus norvegicus | |
0.013 | - |
ferricytochome b5 | wild type enzyme, pH 7.0, 25°C | Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | full length form contains a 3 kDa membrane anchoring domain | Rattus norvegicus | 16020 | - |
soluble | truncated form lacking the membrane anchoring domain, present in erythrocytes | Rattus norvegicus | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
30000 | - |
SDS-PAGE | Rattus norvegicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 ferricytochrome b5 + NADH | Rattus norvegicus | involved in the synthesis of fatty acids and cholesterol, and in the oxidation of xenobiotics, enzyme defects causes methemoglobinemia type I or type II | 2 ferrocytochrome b5 + NAD+ + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | P20070 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzymes | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 ferricyanide + NADH | - |
Rattus norvegicus | 2 ferrocyanide + NAD+ + H+ | - |
? | |
2 ferricytochrome b5 + NADH | - |
Rattus norvegicus | 2 ferrocytochrome b5 + NAD+ + H+ | - |
? | |
2 ferricytochrome b5 + NADH | involved in the synthesis of fatty acids and cholesterol, and in the oxidation of xenobiotics, enzyme defects causes methemoglobinemia type I or type II | Rattus norvegicus | 2 ferrocytochrome b5 + NAD+ + H+ | - |
? | |
NADH + ferricytochrome b5 | - |
Rattus norvegicus | NAD+ + H+ + ferrocytochrome b5 | - |
r | |
NADH + ferricytochrome b5 | - |
Rattus norvegicus | NAD+ + H+ + 2 ferrocytochrome b5 | - |
r |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
33 | - |
ferricytochome b5 | P144L/L148P mutant enzyme, pH 7.0, 25°C | Rattus norvegicus | |
100 | - |
ferricyanide | P144L/L148P mutant enzyme, pH 7.0, 25°C | Rattus norvegicus | |
117 | - |
ferricytochome b5 | P144L mutant enzyme, pH 7.0, 25°C | Rattus norvegicus | |
130 | - |
ferricytochome b5 | L148P mutant enzyme, pH 7.0, 25°C | Rattus norvegicus | |
283 | - |
ferricyanide | P144L mutant enzyme, pH 7.0, 25°C | Rattus norvegicus | |
300 | - |
ferricyanide | L148P mutant enzyme, pH 7.0, 25°C | Rattus norvegicus | |
417 | - |
ferricytochome b5 | wild type enzyme, pH 7.0, 25°C | Rattus norvegicus | |
800 | - |
ferricyanide | wild type enzyme, pH 7.0, 25°C | Rattus norvegicus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | non-covalentely bound prosthetic group | Rattus norvegicus | |
NADH | - |
Rattus norvegicus |