Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | increasing ionic strength, increase in activity | Catharanthus roseus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
cetyltrimethyl ammonium bromide | - |
Catharanthus roseus | |
NADP+ | competitive inhibition | Catharanthus roseus | |
p-chloromercuribenzoate | - |
Catharanthus roseus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0057 | - |
NADPH | - |
Catharanthus roseus | |
0.0078 | - |
ferricytochrome c | - |
Catharanthus roseus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
additional information | - |
Catharanthus roseus | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | stimulation by increasing ionic strength | Catharanthus roseus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Catharanthus roseus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Catharanthus roseus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
purified 120fold by a combination of ion exchange chromatography and gel filtration and 745fold by affinity chromatography on 2',5'-ADP-Sepharose 4B | Catharanthus roseus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
seedling | - |
Catharanthus roseus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.245 | - |
purification by ion exchange chromatography and gel filtration, electron acceptor: dichlorophenolindophenol | Catharanthus roseus |
1.12 | - |
purification by ion exchange chromatography and gel filtration, electron acceptor: ferricyanide | Catharanthus roseus |
1.429 | - |
purification by ion exchange chromatography and gel filtration | Catharanthus roseus |
17.14 | - |
purification by affinity chromatography on 2',5'-ADP-Sepharose 4B | Catharanthus roseus |
22 | - |
in the presence of 0.005 mM FMN | Catharanthus roseus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 ferricytochrome c + NADPH | additional electron acceptor: ferricyanide | Catharanthus roseus | 2 ferrocytochrome c + NADP+ + H+ | - |
? | |
2 ferricytochrome c + NADPH | additional electron acceptor: cytochrome P450 | Catharanthus roseus | 2 ferrocytochrome c + NADP+ + H+ | - |
? | |
2 ferricytochrome c + NADPH | additional electron acceptor: 2,6-dichlorophenolindophenol | Catharanthus roseus | 2 ferrocytochrome c + NADP+ + H+ | - |
? | |
NADPH + H+ + ferricytochrome c | - |
Catharanthus roseus | NADP+ + ferrocytochrome c | - |
r |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Catharanthus roseus | |
FMN | - |
Catharanthus roseus | |
NADPH | - |
Catharanthus roseus |