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Literature summary for 1.6.2.4 extracted from
- Purification and Partial Characterization of NADPH-cytochrome c reductase from Petunia hybrida flowers (1994), Plant Physiol., 106, 643-650.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| additional information | storage of the enzyme at -75°C for 4 months results in a slight activation of the enzyme activity | Petunia x hybrida | |
| potassium phosphate | activity slightly higher than with sodium chloride or sodium sulfate | Petunia x hybrida |  |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| endoplasmic reticulum | - |
Petunia x hybrida | 5783 | - |
| microsome | - |
Petunia x hybrida | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 84000 | - |
Western blot analysis of fresh microsomal extracts | Petunia x hybrida |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Petunia x hybrida | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| using column chromatography on adenosine 2',5'-biphosphate-Sepharose, followed by high-performance anion-exchange chromatography | Petunia x hybrida |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| flower | buds and nascent, open flowers | Petunia x hybrida | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.02 | 0.12 | flower buds, open flowers exhibits a similar value | Petunia x hybrida |
| 60 | 120 | purified enzyme | Petunia x hybrida |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -75°C, months, 5 mM 2'-AMP | Petunia x hybrida |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2 ferricytochrome c + NADPH | additional electron acceptor: neotetrazolium chloride | Petunia x hybrida | 2 ferrocytochrome c + NADP+ + H+ | - |
? | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.4 | - |
- |
Petunia x hybrida |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 8 | - |
Petunia x hybrida |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FAD | - |
Petunia x hybrida | |
| FMN | - |
Petunia x hybrida | |
| NADH | activity not detected | Petunia x hybrida | |
| NADPH | - |
Petunia x hybrida | |
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