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Literature summary for 1.6.3.1 extracted from

  • Kim, H.J.; Chen, C.; Kabbage, M.; Dickman, M.B.
    Identification and characterization of Sclerotinia sclerotiorum NADPH oxidases (2011), Appl. Environ. Microbiol., 77, 7721-7729.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.6.3.1

Activating Compound

Activating Compound Comment Organism Structure
Rac for the activation of Nox enzymes, cytosolic regulatory components (Rac, p67phox, p47phox, and p40phox) are recruited into the integral membrane protein flavocytochrome b558, consisting of the catalytic subunits gp91phox and p22phox Sclerotinia sclerotiorum

Cloned(Commentary)

Cloned (Comment) Organism
genes Ssnox1 and Ssnox2, semiquantitative RT-PCR expression analysis, recombinant expression o f Nox1 and Nox2 in wild-type Sclerotinia sclerotiorum by protoplast transformation Sclerotinia sclerotiorum

Protein Variants

Protein Variants Comment Organism
additional information silencing of genes Ssnox1 and Ssnox2 by siRNA using two silencing vectors (pSNOX1 and pSNOX2) carrying the hygromycin B resistance gene as the selectable marker, defect in sclerotial development of Nox silenced strains, phenotype overview Sclerotinia sclerotiorum

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
 Sclerotinia sclerotiorum 16020
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Sclerotinia sclerotiorum the activated Nox enzyme complex generates superoxide from oxygen by utilizing NADPH as an electron donor, thereby leading to the formation of other species of reactive oxygen ?
-
 ?
additional information Sclerotinia sclerotiorum 1980 the activated Nox enzyme complex generates superoxide from oxygen by utilizing NADPH as an electron donor, thereby leading to the formation of other species of reactive oxygen ?
-
 ?
NADPH + H+ + O2 Sclerotinia sclerotiorum
-
 NADP+ + H2O2
-
 ?
NADPH + H+ + O2 Sclerotinia sclerotiorum 1980
-
 NADP+ + H2O2
-
 ?

Organism

Organism UniProt Comment Textmining
Sclerotinia sclerotiorum
-
-
-
Sclerotinia sclerotiorum 1980
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
sclerotium transcript levels of Nox1 increase in planta and during sclerotial development, transcript levels of Ssnox1 also increase during fungal interaction with plant tissue Sclerotinia sclerotiorum
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the activated Nox enzyme complex generates superoxide from oxygen by utilizing NADPH as an electron donor, thereby leading to the formation of other species of reactive oxygen Sclerotinia sclerotiorum ?
-
 ?
additional information the activated Nox enzyme complex generates superoxide from oxygen by utilizing NADPH as an electron donor, thereby leading to the formation of other species of reactive oxygen Sclerotinia sclerotiorum 1980 ?
-
 ?
NADPH + H+ + O2
-
 Sclerotinia sclerotiorum NADP+ + H2O2
-
 ?
NADPH + H+ + O2
-
 Sclerotinia sclerotiorum 1980 NADP+ + H2O2
-
 ?

Synonyms

Synonyms Comment Organism
NOX1
-
 Sclerotinia sclerotiorum
Nox2
-
 Sclerotinia sclerotiorum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Sclerotinia sclerotiorum

Cofactor

Cofactor Comment Organism Structure
NADPH
-
 Sclerotinia sclerotiorum

Expression

Organism Comment Expression
Sclerotinia sclerotiorum transcript levels of Ssnox1 increase during fungal interaction with plant tissue up

General Information

General Information Comment Organism
malfunction DelTASsnox1 strains have reduced reactive oxygen species levels, are unable to develop sclerotia, and unexpectedly correlate with significantly reduced oxalate production. Inactivation of the Nox2 gene results in limited sclerotial development, but the organism remains fully pathogenic Sclerotinia sclerotiorum
additional information for the activation of Nox enzymes, cytosolic regulatory components (Rac, p67phox, p47phox, and p40phox) are recruited into the integral membrane protein flavocytochrome b558, consisting of the catalytic subunits gp91phox and p22phox Sclerotinia sclerotiorum
physiological function fungal NADPH oxidases are required for pathogenic development and are consistent with the importance of reactive oxygen species regulation in the successful pathogenesis of Sclerotinia sclerotiorum. Central role for SsNox1 in both virulence and pathogenic (sclerotial) development Sclerotinia sclerotiorum