Inhibitors | Comment | Organism | Structure |
---|---|---|---|
5,5'-dithiobis-(2-nitrobenzoate) | 1 mM, 7 min, 50% loss of activity | Pseudothermotoga hypogea | |
CuSO4 | 1 mM, 1 h at 22°C, 85% remaining activity | Pseudothermotoga hypogea | |
FeSO4 | 1 mM, 1 h at 22°C, 56% remaining activity | Pseudothermotoga hypogea | |
HgCl2 | 1 mM, 1 h at 22°C, 6% remaining activity | Pseudothermotoga hypogea | |
MgCl2 | 1 mM, 1 h at 22°C, 93% remaining activity | Pseudothermotoga hypogea | |
NiCl2 | 1 mM, 1 h at 22°C, 71% remaining activity | Pseudothermotoga hypogea | |
ZnSO4 | 1 mM, 1 h at 22°C, 69% remaining activity | Pseudothermotoga hypogea |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0075 | - |
NADH | pH 7.0, 80°C | Pseudothermotoga hypogea | |
0.085 | - |
O2 | pH 7.0, 80°C | Pseudothermotoga hypogea |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
50000 | - |
2 * 50000, SDS-PAGE | Pseudothermotoga hypogea |
100000 | - |
gel filtration | Pseudothermotoga hypogea |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudothermotoga hypogea | - |
- |
- |
Pseudothermotoga hypogea DSM 11164 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Pseudothermotoga hypogea |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
30 | - |
pH 7.0, 80°C | Pseudothermotoga hypogea |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
NADH + H+ + O2 | the enzyme is highly specific for NADH. No formation of H2O | Pseudothermotoga hypogea | NAD+ + H2O2 | - |
? | |
NADH + H+ + O2 | the enzyme is highly specific for NADH. No formation of H2O | Pseudothermotoga hypogea DSM 11164 | NAD+ + H2O2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 50000, SDS-PAGE | Pseudothermotoga hypogea |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
85 | - |
above | Pseudothermotoga hypogea |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
8 h, no loss of activity | Pseudothermotoga hypogea |
90 | - |
100 min, 50% loss of activity | Pseudothermotoga hypogea |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Pseudothermotoga hypogea |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.5 | 7.5 | very active in the pH-range 6.5-7.5 | Pseudothermotoga hypogea |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | a flavoprotein, contains 1.8 mol of non-covalently bound FAD per mol of native enzyme | Pseudothermotoga hypogea | |
NADH | the enzyme is highly specific for NADH | Pseudothermotoga hypogea |
General Information | Comment | Organism |
---|---|---|
physiological function | further investigation is required to conclude firmly if the purified NADH oxidase is part of an enzyme system that protects anaerobic Thermotoga hypogea from accidental exposure to O2 | Pseudothermotoga hypogea |