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Literature summary for 1.6.5.10 extracted from

  • Kitzing, K.; Fitzpatrick, T.B.; Wilken, C.; Sawa, J.; Bourenkov, G.P.; Macheroux, P.; Clausen, T.
    The 1.3 A crystal structure of the flavoprotein YqjM reveals a novel class of Old Yellow Enzymes (2005), J. Biol. Chem., 280, 27904-27913.
    View publication on PubMed
Search for more literature for 1.6.5.10

Crystallization (Commentary)

Crystallization (Comment) Organism
sitting drop vapour diffusion method. In addition to the oxidized and reduced enzyme form, the structures of the complexes with p-hydroxybenzaldehyde and p-nitrophenol, respectively are solved Bacillus subtilis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.019
-
 duroquinone pH 7.5, 25°C Bacillus subtilis

Organism

Organism UniProt Comment Textmining
Bacillus subtilis
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
NADPH + H+ + duroquinone
-
 Bacillus subtilis NADP+ + duroquinol
-
 ?
NADPH + H+ + menadione
-
 Bacillus subtilis NADP+ + reduced menadiol
-
 ?

Subunits

Subunits Comment Organism
tetramer dimer of catalytically dependent dimers Bacillus subtilis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.67
-
 duroquinone pH 7.5, 25°C Bacillus subtilis
3.04
-
 menadione pH 7.5, 25°C Bacillus subtilis

Cofactor

Cofactor Comment Organism Structure
FMN bound non-covalently at the COOH termini of the beta-sheet Bacillus subtilis
NADPH
-
 Bacillus subtilis