Activating Compound | Comment | Organism | Structure |
---|---|---|---|
2-methylene-4-butyrolactone | 25fold induction | Escherichia coli |
Crystallization (Comment) | Organism |
---|---|
- |
Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | insensitive to 4-chloromercuribenzoate at 100 microM, heavy metals such as Cu2+, Co2+, Cd2+ and Mn2+ at 10 microM each, and chelating agents such as EDTA, o-phenanthroline and diethyldithiocarbamate at 100 microM each | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.006 | - |
menadione | pH 7.5, 30°C | Escherichia coli | |
0.0105 | - |
NADPH | pH 7.5, 30°C | Escherichia coli |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
soluble | - |
Escherichia coli | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
additional information | - |
enzyme displays three charge isomers with different relative mobilities | Escherichia coli |
21000 | - |
2 * 21000, SDS-PAGE | Escherichia coli |
42000 | - |
PAGE | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1067 | - |
pH 7.5, 30°C | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1,4-benzoquinone + NADPH + H+ | about 81% of the activity with menadione | Escherichia coli | 1,4-benzoquinol + NADP+ | - |
? | |
1,4-naphthoquinone + NADPH + H+ | about 85% of the activity with menadione | Escherichia coli | 1,4-naphthoquinol + NADP+ | - |
? | |
2 ferricyanide + NADPH | about 90% of the activity with menadione | Escherichia coli | 2 ferrocyanide + NADP+ + H+ | - |
? | |
dichlorophenolindophenol + NADPH + H+ | about 15% of the activity with menadione | Escherichia coli | ? + NADP+ | - |
? | |
menadione + NADPH + H+ | reduction follows a two-electron-pathway | Escherichia coli | menadiol + NADP+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 21000, SDS-PAGE | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | enzyme displays flavin absorption spectrum with peaks at 376 and 456 nm and a shoulder at around 484 nm. Protein caontains FAD | Escherichia coli | |
additional information | no cofactor: NADH, FMN | Escherichia coli | |
NADPH | specific | Escherichia coli |