Literature summary for 1.6.5.2 extracted from

Sanchez, L.B.; Elmendorf, H.; Nash, T.E.; Muller, M.
NAD(P)H:menadione oxidoreductase of the amitochondriate eukaryote Giardia lamblia: a simpler homologue of the vertebrate enzyme (2001), Microbiology, 147, 561-570.

Search for more literature for 1.6.5.2

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Giardia intestinalis

Inhibitors

Inhibitors	Comment	Organism	Structure
1,10-phenanthroline	-	Giardia intestinalis
3,3'-methylene-bis(4-hydroxycoumarin)	-	Giardia intestinalis
N-ethylmaleimide	-	Giardia intestinalis
quercetin	-	Giardia intestinalis
quinacrine dihydrochloride	0.05 mM, 65-95% inhibition depending on substrate	Giardia intestinalis

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.000188	-	menadione	cofactor NADH	Giardia intestinalis
0.00024	-	menadione	cofactor NADPH	Giardia intestinalis
0.000437	-	naphthoquinone	cofactor NADH	Giardia intestinalis
0.00059	-	naphthoquinone	cofactor NADPH	Giardia intestinalis
0.03	-	NADPH	reduction of menadione	Giardia intestinalis
0.033	-	NADPH	reduction of naphthoquinone	Giardia intestinalis
0.045	-	NADH	reduction of naphthoquinone	Giardia intestinalis
0.549	-	NADH	reduction of menadione	Giardia intestinalis

Organism

Organism	UniProt	Comment	Textmining
Giardia intestinalis	-	amitochondriate eukaryote	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant enzyme	Giardia intestinalis

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
1,4-naphthoquinone + NAD(P)H	-	Giardia intestinalis	1,4-naphthohydroquinone + NAD(P)+	-	?
2-methyl-1,4-naphthoquinone + NAD(P)H	-	Giardia intestinalis	2-methyl-1,4-naphthoquinol + NAD(P)+	-	?
3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide + NAD(P)H	-	Giardia intestinalis	reduced 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide + NAD(P)+	-	?
K3[Fe(CN)6] + NAD(P)H	-	Giardia intestinalis	K4[Fe(CN)6] + NAD(P)+	-	?
menadione + NADH + H+	-	Giardia intestinalis	menadiol + NAD+	-	r
methyl red + NAD(P)H	-	Giardia intestinalis	reduced methyl red + NAD(P)+	-	?
naphthoquinone + NADH + H+	-	Giardia intestinalis	naphthoquinol + NAD+	-	r

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
23.1	-	menadione	cofactor NADH	Giardia intestinalis
29.9	-	naphthoquinone	cofactor NADH	Giardia intestinalis
31.7	-	NADH	reduction of menadione	Giardia intestinalis
34.8	-	menadione	cofactor NADPH	Giardia intestinalis
39.3	-	naphthoquinone	cofactor NADPH	Giardia intestinalis
47.5	-	NADPH	reduction of naphthoquinone	Giardia intestinalis
52.7	-	NADPH	reduction of menadione	Giardia intestinalis
62.7	-	NADH	reduction of naphthoquinone	Giardia intestinalis

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
0.00095	-	dicoumarol	cofactor NADH	Giardia intestinalis
0.00109	-	dicoumarol	cofactor NADH	Giardia intestinalis
0.00412	-	dicoumarol	cofactor NADPH	Giardia intestinalis
0.00511	-	dicoumarol	cofactor NADPH	Giardia intestinalis

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Release 2023.1 (January 2023)